Abstract
Nano-sized copper particles are widely used in various chemical, physical, and biological fields. However, earlier studies have shown that nano copper particles (40-100 μg/mL) can induce cell toxicity and apoptosis. Therefore, this study was conducted to investigate the role of nano copper in mitochondrion-mediated apoptosis in PK-15 cells. The cells were treated with different doses of nano copper (20, 40, 60, and 80 μg/mL) to determine the effects of apoptosis using acridine orange/ethidium bromide (AO/EB) fluorescence staining and a flow cytometry assay. The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the PK-15 cells were examined using commercially available kits. Moreover, the mRNA levels of the Bax, Bid, Caspase-3, and CYCS genes were assessed by real-time PCR. The results revealed that nano copper exposure induced apoptosis and changed the mitochondrial membrane potential. In addition, nano copper significantly altered the levels of the Bax, Bid, Caspase-3, and CYCS genes at a concentration of 40 μg/mL. To summarize, nano copper significantly (P < 0.05) decreased the level of SOD and increased the level of MDA in PK-15 cells. Altogether, these results suggest that nano copper can play an important r...Continue Reading
References
Oct 1, 1984·Toxicology and Applied Pharmacology·C L MirandaD R Buhler
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·J D RothsteinR W Kuncl
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·C M Troy, M L Shelanski
Jan 1, 1997·The American Journal of Clinical Nutrition·J R TurnlundT M Sakanashi
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·C HertelJ A Kemp
Aug 29, 1998·Journal of Neurochemistry·M Patel
Sep 30, 1998·Cell·H YoshidaT W Mak
Aug 12, 1999·Archives of Biochemistry and Biophysics·M InoueJ Sasaki
Jun 6, 2000·Mutation Research·J Termini
Sep 17, 2002·Cell Death and Differentiation·G van LooP Vandenabeele
Oct 29, 2002·Chemistry & Biology·Ziwei Huang
Jan 16, 2003·The Science of the Total Environment·Björn P ZietzHartmut Dunkelberg
May 23, 2003·Cell Death and Differentiation·E GottliebC B Thompson
Jun 28, 2003·FEMS Microbiology Reviews·Christopher Rensing, Gregor Grass
Dec 23, 2004·Journal of Cardiovascular Pharmacology·Peter DeresKalman Toth
Jan 27, 2005·Cancer Letters·Susanne U Mertens-Talcott, Susan S Percival
Nov 18, 2005·Toxicology Letters·Zhen ChenLijun Wan
Feb 4, 2006·Science·Andre NelNing Li
Aug 2, 2006·Internal Medicine·Hiroyuki Tsutsui
Nov 21, 2007·Toxicology Letters·Huan MengYuliang Zhao
Jan 15, 2008·Free Radical Biology & Medicine·Scott K PowersAndreas N Kavazis
Aug 19, 2008·Toxicology and Applied Pharmacology·Ronghui LeiMingyang Liao
Sep 27, 2008·Journal of Clinical Biochemistry and Nutrition·Mikinori KuwabaraOsamu Inanami
Mar 31, 2011·Nanotoxicology·Susana I L GomesMónica J B Amorim
Jul 12, 2011·Folia Histochemica Et Cytobiologica·Chui-Feng FanEn-Hua Wang
Oct 18, 2011·Toxicology·Abhijit SarkarParames C Sil
Aug 21, 2012·Archives of Toxicology·Jing XuZhuo Yang
Oct 16, 2012·Journal of Hazardous Materials·Pengjuan XuZhuo Yang
Jan 28, 2014·Cell Death and Differentiation·D BrasacchioJ A Trapani
Jun 30, 2014·Biological Trace Element Research·Cui-Yan ZhaoZhao-Xin Tang
Jun 17, 2016·Poultry Science·Katarzyna OgnikKrzysztof Kozłowski
Citations
Feb 8, 2018·Scientific Reports·Khalid MehmoodJiakui Li
May 1, 2019·Environmental Science and Pollution Research International·Yali ZhaoDonghai Zhou
Mar 23, 2018·Environmental Science and Pollution Research International·Xinxin TianDonghai Zhou
Jun 20, 2019·Nutrients·Ludmila V PuchkovaRoman S Polishchuk
Jun 26, 2020·International Journal of Nanomedicine·Badr E El BialyWael N Hozzein
Mar 9, 2019·Animals : an Open Access Journal From MDPI·Xiong JiangJiakui Li
Aug 5, 2018·Environmental Science and Pollution Research International·Hui ZhangJiakui Li
Dec 23, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A Solorio-RodríguezA De Vizcaya-Ruiz
Feb 8, 2021·Ecotoxicology and Environmental Safety·Wenlan YuZhaoxin Tang
Jun 3, 2020·Molecular Pharmaceutics·Barbara CanonicoSandra García-Gallego