Nano-fibrin stabilized CaSO4 crystals incorporated injectable chitin composite hydrogel for enhanced angiogenesis & osteogenesis

Carbohydrate Polymers
R Arun KumarR Jayakumar

Abstract

Calcium sulfate (CaSO4), an excellent biodegradable bone forming agent that is an ideal choice as additive in gels, however, its disadvantage being poor gel rheology and angiogenesis. Here, we have synthesized chitin-CaSO4-nano-fibrin based injectable gel system which shows improved rheology and angiogenic potential. Rheological studies showed that the composite gel was a shear thinning gel with elastic modulus of 15.4±0.275kPa; a 1.67 fold increase over chitin control. SEM and XRD analyses revealed the effect of nano-fibrin (nFibrin) in transforming CaSO4 crystal shape from needle to hexagonal. It also masked the retarding effect of CaSO4 towards in vitro early cell attachment and angiogenesis using rabbit adipose derived mesenchymal stem cells (rASCs) and HUVECs, respectively. rASCs osteogenesis was confirmed by spectrophotometric endpoint assay, which showed 6-fold early increase in alkaline phosphatase levels and immuno-cytochemistry analysis. These in vitro results highlight the potential of injectable chitin-CaSO4-nFibrin gel for osteo-regeneration via enhanced angiogenesis.

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Citations

Jun 15, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marius RădulescuMariana Carmen Chifiriuc
Nov 25, 2017·Advanced Healthcare Materials·Hwan D KimNathaniel S Hwang
Oct 11, 2019·Journal of Materials Science. Materials in Medicine·Marta Calvo CatoiraFrancesca Boccafoschi
Jun 16, 2016·Biomedical Materials·M Vishnu PriyaR Jayakumar
Jan 11, 2020·Journal of Nanobiotechnology·Wenjing LiuLongquan Shao
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Sivashanmugam AmirthalingamJayakumar Rangasamy
Oct 1, 2018·Progress in Polymer Science·Shengjie LingDavid L Kaplan
Oct 20, 2020·Journal of Oral Biology and Craniofacial Research·Divya MehrotraR K Singh
May 3, 2020·Current Stem Cell Research & Therapy·Lang WangJingang Xiao

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