Nano-hydroxyapatite in oral care cosmetics: characterization and cytotoxicity assessment

Scientific Reports
Catarina C CoelhoMaria H Fernandes

Abstract

Nano-hydroxyapatite has been used as an oral care ingredient, being incorporated in several products for the treatment of dental hypersensitivity and enamel remineralisation. Despite its promising results, regulatory and safety concerns have been discussed and questioned by the European Scientific Committee on Consumer Safety (SCCS) regarding the usage of hydroxyapatite nanoparticles in oral care products. In this work, a commercially available nano-hydroxyapatite was characterized and its cytocompatibility towards human gingival fibroblasts was evaluated, as well as its irritation potential using the in vitro HET-CAM assay. All the conditions chosen in this study tried to simulate the tooth brushing procedure and the hydroxyapatite nanoparticles levels normally incorporated in oral care products. The commercial hydroxyapatite nanoparticles used in this study exhibited a rod-like morphology and the expected chemical and phase composition. The set of in vitro cytotoxicity parameters accessed showed that these nanoparticles are highly cytocompatible towards human gingival fibroblasts. Additionally, these nanoparticles did not possess any irritation potential on HET-CAM assay. This study clarifies the issues raised by SCCS and it ...Continue Reading

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Citations

May 13, 2020·Drug Metabolism Reviews·Ioana Roxana BordeaDoina Adina Todea
Dec 5, 2019·International Journal of Molecular Sciences·Chih-Hsiang FangZwei-Chieng Chang
May 24, 2020·Nanomaterials·Georgios FytianosGeorge Z Kyzas
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Jan 20, 2021·ACS Biomaterials Science & Engineering·Zuzanna BuchwaldAdam Voelkel
Jan 19, 2021·ACS Biomaterials Science & Engineering·Farzad ForoutanDaniela Carta

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Methods Mentioned

BETA
x-ray diffraction
flow cytometry
Assay

Software Mentioned

ImageJ Fiji
Zeiss
Gen5
FlowJo
AxioVision
GraphPad Prism

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