Nano-mechanical reinforcement in drug-resistant ovarian cancer cells

Biological & Pharmaceutical Bulletin
Young Ho SeoSoyeun Park

Abstract

The mechanical properties of cells are considered promising biomarkers for the early detection of cancer and the testing of drug efficacy against it. Nevertheless, generalized correlations between drug resistance and the nano-mechanical properties of cancer cells are yet to be defined due to the lack of necessary studies. In this study, we conducted atomic force microscopy (AFM)-based nano-mechanical measurements of cisplatin-sensitive (A2780) and cisplatin-resistant (A2780cis) ovarian cancer cells. The difference in the efficacy of cisplatin between A2780 and A2780cis was confirmed in the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. We observed that the cisplatin-resistant ovarian cancer cells were more motile than cisplatin-sensitive cells based on the results of the wound closure experiment, and the AFM experiments showed that drug resistance induced nano-mechanical stiffening of the ovarian cancer cells. Increased mechanical stiffness caused by cisplatin resistance was consistent with the confocal microscopy images showing more distinct actin stress fibers in A2780cis than in A2780 cells. The down regulation of vinculin implicated the actin-driven elongation as a majo...Continue Reading

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Citations

Sep 2, 2016·Biological & Pharmaceutical Bulletin·Soyeun ParkChul-Ho Jeong
May 17, 2018·British Journal of Haematology·Susanne Bram EdnerssonPer-Ola Andersson
Aug 2, 2018·Biological Chemistry·Collins Otieno Nyongesa, Soyeun Park
Jun 10, 2016·International Journal of Oncology·Jing LiJian Zhang
Oct 18, 2020·Research in Veterinary Science·Ali Raza Jahejo, Wen Xia Tian
Nov 5, 2019·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Luca AndreiMilica Pešić
Nov 26, 2020·International Journal of Molecular Sciences·Andrzej KubiakMałgorzata Lekka
May 1, 2021·Cells·Gaël RunelIngrid Masse
Feb 23, 2020·Biochemical and Biophysical Research Communications·Chongyu ZhaoLinyan Zhu

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