Nano-mole scale sequential signal assignment by (1)H-detected protein solid-state NMR

Chemical Communications : Chem Comm
Songlin WangYoshitaka Ishii

Abstract

We present a 3D (1)H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10-100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D (13)C-detected SSNMR approach.

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Citations

Jan 24, 2016·The Journal of Chemical Physics·Rongchun Zhang, Ayyalusamy Ramamoorthy
Oct 20, 2015·Solid State Nuclear Magnetic Resonance·Ayesha WickramasingheYoshitaka Ishii
Nov 7, 2015·Chemical Communications : Chem Comm·Natalia KulminskayaRasmus Linser
Apr 22, 2016·Angewandte Chemie·Hugh R W DannattGuido Pintacuda
Jun 17, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Rongchun ZhangAyyalusamy Ramamoorthy
Aug 5, 2016·Proceedings of the National Academy of Sciences of the United States of America·Loren B AndreasGuido Pintacuda
Sep 28, 2016·Angewandte Chemie·João Medeiros-SilvaMarkus Weingarth
Jan 29, 2021·Solid State Nuclear Magnetic Resonance·Yi JiGuangjin Hou
Dec 11, 2017·Journal of Magnetic Resonance·Yoshitaka IshiiTakayuki Kamihara
Aug 3, 2018·The Journal of Physical Chemistry. B·Piotr PaluchMarek J Potrzebowski
Nov 27, 2019·Analytical Chemistry·Enping LinZhong Chen
May 26, 2017·The Journal of Physical Chemistry. B·Rongchun ZhangAyyalusamy Ramamoorthy

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