PMID: 22572182Jan 1, 2010Paper

Nano-scale analyses of the chromatin decompaction induced by histone acetylation

Archives of Histology and Cytology
Kohji HizumeKunio Takeyasu

Abstract

The acetylation of histone tails is a key factor in the maintenance of chromatin dynamics and cellular homeostasis. The hallmark of active chromatin is the hyper-acetylation of histones, which appears to result in a more open chromatin structure. Although short nucleosomal arrays have been studied, the structural dynamics of relatively long acetylated chromatin remain unclear. We have analyzed in detail the structure of long hyper-acetylated chromatin fibers using atomic force microscopy (AFM). Hyper-acetylated chromatin fibers isolated from nuclei that had been treated with Trichostatin A (TSA), an inhibitor of histone deacetylase, were found to be thinner than those from untreated nuclei. The acetylated chromatin fibers were more easily spread out of nuclei by high-salt treatment, implying that hyper-acetylation facilitates the release of chromatin fibers from compact heterochromatin regions. Chromatin fibers reconstituted in vitro from core histones and linker histone H1 became thinner upon acetylation. AFM imaging indicated that the gyration radius of the nucleosomal fiber increased after acetylation and that the hyper-acetylated nucleosomes did not aggregate at high salt concentrations, in contrast to the behavior of non-a...Continue Reading

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Citations

Dec 12, 2012·Bioscience, Biotechnology, and Biochemistry·Eloise PrietoKunio Takeyasu
Aug 23, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Marina KrauseKatarina Wolf
Jun 26, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kohji HizumeHiroyuki Araki
Oct 13, 2020·Frontiers in Cell and Developmental Biology·Claudia Tanja Mierke

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