Nano to micro -- fluorescence measurements of electric fields in molecules and genetically specified neurons.

The Journal of Membrane Biology
Rikard BlunckFrancisco Bezanilla

Abstract

Our central nervous system is based on the generation and propagation of electrical signals along the neuronal pathways. These variations of the membrane potential are arranged by the concerted action of ion channels in the neuronal membrane. Therefore, the exact measurement of the electric field in the central nervous system is the focus of intensive investigation. While electrophysiological methods provide exact measurements on the single-cell or single-molecule level with high temporal resolution, they are limited in their spatial resolution ranging from a few single cells to a single molecule. To thoroughly understand how the voltage-dependent ion channels sense the membrane potential and are precisely gated by it, the electric field within the protein has to be investigated. Likewise, the propagation of electrical impulses in a network of neurons involves a large number of cells, which have to be monitored simultaneously. For these endeavors, optical methods have proven to be useful due to their scalability, temporal and spatial resolution. Here, we will summarize the properties of the optical probes that we used to determine the electrical field strength within voltage-sensitive ion channels and discuss the hybrid approac...Continue Reading

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Citations

Apr 15, 2010·Journal of Fluorescence·Magda PrzybyloMarek Langner
Nov 30, 2006·The Journal of General Physiology·Donald D F LooErnest M Wright
May 21, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amanda FoustDavid A McCormick
Jul 25, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jonathan BradleyDavid A DiGregorio
Dec 16, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amanda E Casale, David A McCormick
Jun 2, 2009·Chemistry and Physics of Lipids·Alexander P Demchenko, Semen O Yesylevskyy
Oct 16, 2015·Nature Reviews. Neuroscience·David Holcman, Rafael Yuste
Jan 12, 2011·Neuron·Darcy S PeterkaRafael Yuste
Dec 23, 2017·Nanoscale·Maria Tsemperouli, Kaori Sugihara
Jul 1, 2021·Journal of the American Chemical Society·Yuecheng ZhouBianxiao Cui

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