Nanoarchaeota, Their Sulfolobales Host, and Nanoarchaeota Virus Distribution across Yellowstone National Park Hot Springs

Applied and Environmental Microbiology
Jacob H Munson-McGeeMark J Young

Abstract

Nanoarchaeota are obligate symbionts with reduced genomes first described from marine thermal vent environments. Here, both community metagenomics and single-cell analysis revealed the presence of Nanoarchaeota in high-temperature (∼90°C), acidic (pH ≈ 2.5 to 3.0) hot springs in Yellowstone National Park (YNP) (United States). Single-cell genome analysis of two cells resulted in two nearly identical genomes, with an estimated full length of 650 kbp. Genome comparison showed that these two cells are more closely related to the recently proposed Nanobsidianus stetteri from a more neutral YNP hot spring than to the marine Nanoarchaeum equitans. Single-cell and catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) analysis of environmental hot spring samples identified the host of the YNP Nanoarchaeota as a Sulfolobales species known to inhabit the hot springs. Furthermore, we demonstrate that Nanoarchaeota are widespread in acidic to near neutral hot springs in YNP. An integrated viral sequence was also found within one Nanoarchaeota single-cell genome and further analysis of the purified viral fraction from environmental samples indicates that this is likely a virus replicating within the YNP Nanoarchaeota.

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Citations

Mar 3, 2018·Genes·Jacob H Munson-McGeeMark J Young
Jan 11, 2019·FEMS Microbiology Letters·Nina DombrowskiAnja Spang
Feb 16, 2019·Environmental Microbiology Reports·Emily St JohnAnna-Louise Reysenbach
Nov 1, 2017·Nature Methods·Tanja WoykeFrederik Schulz
Mar 25, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Matthew D PaulyRachel J Whitaker
Aug 26, 2020·The ISME Journal·Daniel R UtterBatbileg Bor
Mar 23, 2017·Frontiers in Microbiology·Trevor Nicks, Lilah Rahn-Lee
Sep 7, 2018·Frontiers in Microbiology·Lydia-Ann J GhuneimOlga V Golyshina
Aug 18, 2017·Antonie van Leeuwenhoek·Andrey V MardanovNikolai V Ravin
Feb 3, 2020·Cellular and Molecular Life Sciences : CMLS·István Zachar, Gergely Boza
Jan 18, 2021·The Science of the Total Environment·Verónica MolinaMartha Hengst
Feb 13, 2019·Angewandte Chemie·Jackson K B Cahn, Jörn Piel

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