Nanobody-Directed Specific Degradation of Proteins by the 26S-Proteasome in Plants

Frontiers in Plant Science
Bianca BaudischUdo Conrad

Abstract

Here, we present data showing the directed degradation of target proteins recognized by a specific nanobody in transgenic plants. Green fluorescent protein was depleted by a chimeric nanobody fused to a distinct F-box domain, which enables protein degradation via the ubiquitin proteasome pathway. This technique could thus be used to knock out other proteins of interest in planta using specific, high-affinity binding proteins.

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Citations

Dec 21, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kamila KalinowskaAndreas Houben
Nov 5, 2019·Frontiers in Genetics·Salvatrice CiccareseRachele Antonacci
Jul 3, 2019·Wiley Interdisciplinary Reviews. Developmental Biology·Gustavo AguilarShinya Matsuda
Oct 11, 2020·Plant Molecular Biology·Wenyi WangChangan Jiang
Oct 6, 2020·Frontiers in Plant Science·Kenji MiuraYukinari Kato
Dec 2, 2020·Biology·Yuri ProzzilloGiovanni Messina
Dec 3, 2020·American Journal of Physiology. Cell Physiology·Jan Gettemans, Brian De Dobbelaer

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Methods Mentioned

BETA
phage display
transgenic
PCR
ELISA

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