Nanocolloidal gold-based immunoassay for the detection of the N-methylcarbamate pesticide carbofuran

Journal of Agricultural and Food Chemistry
Pei ZhouAngel Montoya

Abstract

Nanocolloidal gold particles were prepared and labeled to an anti-carbofuran monoclonal antibody (Mab). This conjugate was dispensed on the conjugated pad of a porous glass fiber. Ovalbumin (OVA)-carbofuran and goat anti-mouse IgG were dispensed on the nitrocellulose (NC) membrane and served as the test line and control line, respectively. The carbofuran-containing sample migrated to the NC membrane and reacted with the anti-carbofuran Mab labeled with the colloidal gold. The mixture diffused along the membrane and passed through the OVA-carbofuran in the test line via capillary action. The more analyte present in the sample, the more effectively it will compete with the carbofuran immobilized on the test line for binding to the limited amount of antibody labeled with colloidal gold. An adequate amount of carbofuran could prevent attachment of the colored conjugate to the test line. The presence or absence of a colored band on the test line could indicate a negative or positive result, respectively. When measured to the water sample spiked with carbofuran, this was obtained at or above 0.25 mg/L of carbofuran. The major advantages of the one-step strip test are that the detection time needed was <10 min and all of the reagents ...Continue Reading

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Citations

Jul 14, 2006·Analytical and Bioanalytical Chemistry·Ryo TanakaEiichi Tamiya
Sep 3, 2013·Journal of Immunological Methods·Cebrail Karakus, Barik A Salih
Aug 13, 2008·Analytical and Bioanalytical Chemistry·Geertruida A Posthuma-TrumpieAart van Amerongen
Dec 6, 2006·Analytical and Bioanalytical Chemistry·Anna Yu KolosovaCarlos Van Peteghem
Nov 3, 2006·Biomedical Chromatography : BMC·Xu ChuanlaiLiu Liqiang
Jun 10, 2011·Proteomics. Clinical Applications·Tao WangHuaizhong Hu
Aug 26, 2014·Journal of Immunoassay & Immunochemistry·Cebrail Karakus
Jul 19, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Xi-Chun WangJia-Sheng Wang
Dec 2, 2011·Chemical Society Reviews·Lev Dykman, Nikolai Khlebtsov
Oct 8, 2019·Journal of Food Science·Yajie ZhaoBaomin Wang
Apr 9, 2008·Journal of Agricultural and Food Chemistry·Yiqiang ChenXilong Xiao

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