Nanocomplexes from RGD-modified generation 1.0 polyamidoamine based copolymers used for intravascular gene release to prevent restenosis

Nanomedicine
Duanwen CaoShirong Pan

Abstract

To validate the efficacy of nanocomplexes from RGD-modified polyamidoamine (PAMAM G1) copolymer for prevention of restenosis. Generation 1.0 polyamidoamine (PAMAM G1)-based copolymers (PGP) and RGD modified PGP (PGP-RGD) were synthesized and its properties were evaluated. Intravascular VEGF165 release tests were performed. The PGP-RGD1 (2.6% grafting rate) exhibited lower cytotoxicity and larger combining ability with pDNA. The complexes had sizes of 80-160 nm and zeta potentials of 3-20 mV. Transfection efficiency of PGP-RGD1 complexes in human umbilical vein endothelial cells was larger than that of PGP complexes. Patency and expression level of artery in PGP-RGD1 group were higher than that in saline group. PGP-RGD1 will be a promising targeted gene vector.

References

Nov 25, 2005·Advanced Drug Delivery Reviews·Sönke Svenson, Donald A Tomalia
Nov 25, 2005·Chemical Communications : Chem Comm·Rameshwer ShuklaJames R Baker Jr
Nov 29, 2005·Advanced Drug Delivery Reviews·Christine DufèsAndreas G Schätzlein
Oct 14, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Jing YangXiaohui Li
Sep 25, 2009·Bioconjugate Chemistry·Carolyn L Waite, Charles M Roth
Oct 17, 2009·The AAPS Journal·Mohammed S Al-Dosari, Xiang Gao
May 4, 2010·International Journal of Pharmaceutics·Keerti JainN K Jain
Feb 2, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Huayu TianAtsushi Maruyama
Feb 26, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Neha ShahHarendra S Parekh
Apr 19, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Lihong ZhangYanyan Jiang
Jul 23, 2011·Journal of Pharmaceutical Sciences·Tomoaki KurosakiHitoshi Sasaki
Sep 7, 2011·International Journal of Pharmaceutics·Shirong PanMin Feng
May 17, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ming ShenVictor C Yang
Jul 18, 2012·International Journal of Biological Macromolecules·Kishor Sarkar, P P Kundu
Sep 4, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Min HanJian-Qing Gao
Oct 12, 2012·Journal of the American Chemical Society·Hongmei LiuYiyun Cheng
Oct 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shirong PanMin Feng

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
transfection
Assay
ELISA
flow cytometry
percutaneous transluminal coronary angioplasty
protein assay

Software Mentioned

Micro
- Image Analysis System

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.