Dec 21, 2016

Nanocontact Printing of Proteins on Physiologically Soft Substrates to Study Cell Haptotaxis

Langmuir : the ACS Journal of Surfaces and Colloids
Donald MacNearneyDavid Juncker


Surface bound guidance cues and gradients are vital for directing cellular processes during development and repair. In vivo, these cues are often presented within a soft extracellular matrix with elastic moduli E < 10 kPa, but in vitro haptotaxis experiments have been conducted primarily on hard substrates with elastic moduli in the MPa to GPa range. Here, a technique is presented for patterning haptotactic proteins with nanometer resolution on soft substrates with physiological elasticity. A new nanocontact printing process was developed that circumvented the use of plasma activation that was found to alter the mechanical properties of the substrate. A dissolvable poly(vinyl alcohol) film was first patterned by lift-off nanocontact printing, and in turn printed onto the soft substrate, followed by dissolution of the film in water. An array of 100 unique digital nanodot gradients (DNGs), consisting of millions of 200 × 200 nm2 protein nanodots, was patterned in less than 5 min with with <5% average deviation from the original gradient design. DNGs of netrin-1, a known protein guidance cue, were patterned, and the unpatterned surface was backfilled with a reference surface consisting of 75% polyethylene glycol grafted with polyl...Continue Reading

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Mentioned in this Paper

In Vivo
Cellular Process
Occlusal Guidance
Gene Products, Protein
Extracellular Matrix
Neutrophils as Percentage of Blood Leukocytes (Lab Test)

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