Nanocrystal biolabels with releasable fluorophores for immunoassays

Analytical Chemistry
Cangel Pui-yee ChanReinhard Renneberg

Abstract

A novel signal amplification technology based on a new class of biofunctional fluorescent nanocrystals holds promise to improve the sensitivity and the limits of detection of immunoassays. A two-step approach without layer-by-layer techniques is described to encapsulate the fluorogenic precursor fluorescein diacetate (FDA) nanocrystals (107-nm average size) followed by conjugation of the antibody. Distearoylphosphatidylethanolamine (DSPE) modified with amino(poly(ethylene glycol)) (PEG(2000)Amine) is coated on the surface of the FDA nanocrystals to provide a interface for the antibody coupling. Anti-mouse antibodies are attached to the nanocrystalline FDA biolabels by adsorption. A high molar ratio of fluorescent molecules to biomolecules (2.8 x 10(4)) is achieved in this nanocrystal biolabel system. The analytical performance of the nanocrystal-based label system is evaluated in a model sandwich immunoassay for the detection of mouse IgG. After separation of the nonbound antibody nanocrystal labels, fluorophores are released by hydrolysis and dissolution of the nanocrystalline FDA. Due to the release of the fluorophores (fluoresceins) into a large volume of organic solvent/sodium hydroxide mixture, self-quenching is suppressed...Continue Reading

References

Jun 17, 1999·International Journal of Pharmaceutics·G BendasU Rothe
Sep 25, 2001·Analytical Chemistry·Y Lvov, F Caruso

❮ Previous
Next ❯

Citations

Jan 6, 2006·Analytical and Bioanalytical Chemistry·King-Keung SinReinhard Renneberg
Mar 10, 2010·Journal of Fluorescence·Thomas BehnkeUte Resch-Genger
Aug 30, 2008·Nature Methods·Ute Resch-GengerThomas Nann
Apr 1, 2009·Bioanalysis·Cangel Pui-Yee Chan
Jul 1, 2010·Therapeutic Delivery·Evren H GokceEliana B Souto
Mar 27, 2013·Annual Review of Analytical Chemistry·Cangel Pui Yee ChanReinhard Renneberg
Jan 1, 2010·Expert Opinion on Medical Diagnostics·Alfredo de la Escosura-Muñiz, Arben Merkoçi
Feb 24, 2007·Trends in Biotechnology·Paolo FortinaScott A Waldman
Apr 28, 2005·Biosensors & Bioelectronics·Matthias Seydack
Apr 29, 2008·IEEE Transactions on Bio-medical Engineering·Janice J LiGregory T A Kovacs
Jan 4, 2005·Journal of Immunological Methods·Cangel Pui-Yee ChanReinhard Renneberg
Jul 9, 2013·Analytical Biochemistry·Sara NouraniSeyed Mehdi Boutorabi
Oct 24, 2014·Analytical and Bioanalytical Chemistry·C WürthU Resch-Genger
Aug 29, 2007·Analytica Chimica Acta·Lin He, Chee-Seng Toh
Jun 27, 2006·IEE Proceedings. Nanobiotechnology·K-K SinR Renneberg
Oct 3, 2006·Expert Review of Molecular Diagnostics·Subhash Dhawan
Mar 7, 2020·Bioengineered·Khalisanni KhalidLai Chin Wei
Dec 14, 2011·Nanoscale·A PatraT P Radhakrishnan
Jan 27, 2015·Angewandte Chemie·Kai HiltebrandtChristopher Barner-Kowollik
Jan 24, 2009·Analytical Chemistry·Dongxuan Shen, Mark E Meyerhoff
Mar 28, 2007·Analytica Chimica Acta·Cangel Pui-yee ChanMatthias Seydack
Feb 22, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Xinhua HuangIl Kim
Oct 15, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Changchun ZhengYunqi Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Analytical and Bioanalytical Chemistry
King-keung SinReinhard Renneberg
Journal of Immunological Methods
Cangel Pui-yee ChanReinhard Renneberg
© 2022 Meta ULC. All rights reserved