Nanocrystal facet modulation to enhance transferrin binding and cellular delivery.

Nature Communications
Yu QiWei Chen

Abstract

Binding of biomolecules to crystal surfaces is critical for effective biological applications of crystalline nanomaterials. Here, we present the modulation of exposed crystal facets as a feasible approach to enhance specific nanocrystal-biomolecule associations for improving cellular targeting and nanomaterial uptake. We demonstrate that facet-engineering significantly enhances transferrin binding to cadmium chalcogenide nanocrystals and their subsequent delivery into cancer cells, mediated by transferrin receptors, in a complex biological matrix. Competitive adsorption experiments coupled with theoretical calculations reveal that the (100) facet of cadmoselite and (002) facet of greenockite preferentially bind with transferrin via inner-sphere thiol complexation. Molecular dynamics simulation infers that facet-dependent transferrin binding is also induced by the differential affinity of crystal facets to water molecules in the first solvation shell, which affects access to exposed facets. Overall, this research underlines the promise of facet engineering to improve the efficacy of crystalline nanomaterials in biological applications.

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Citations

Sep 29, 2020·EMBO Reports·Emma L Clayton, Adrian M Isaacs
Aug 21, 2021·Journal of Hazardous Materials·Dandan LiJiwei Luo

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Methods Mentioned

BETA
X-ray
fluorescence microscopy
transmission electron microscopy
confocal microscopy
Assay
Protein Assay

Software Mentioned

VMD
GROMACS
Syngistix
Statistical Product and Service Solutions ( SPSS
Vienna Ab - initio Simulation Package ( VASP )
Mascot Search
Mascot

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