Nanodroplet real-time PCR system with laser assisted heating.

Optics Express
Hanyoup KimGregory W Faris

Abstract

We report the successful application of low-power (approximately 30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature measurement, and Taqman real-time readout in nanoliter droplets on a disposable plastic substrate. A selective heating scheme using an infrared laser appears ideal for driving PCR because it heats only the droplet, not the oil or plastic substrate, providing fast heating and completing the 40 cycles of PCR in 370 seconds. No microheaters or microfluidic circuitry were deposited on the substrate, and PCR was performed in one droplet without affecting neighboring droplets. The assay performance was quantitative and its amplification efficiency was comparable to that of a commercial instrument.

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Citations

Apr 17, 2009·Lab on a Chip·Hanyoup KimGregory W Faris
Apr 7, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Sanhita S DixitGregory W Faris
Apr 25, 2014·IEEE Transactions on Bio-medical Engineering·Seung-Min ParkLuke P Lee
Dec 3, 2013·Analytical Biochemistry·Lindsay N Sanford, Carl T Wittwer
Apr 1, 2015·PloS One·Michael S BartschKamlesh D Patel
Feb 11, 2012·Analytical Chemistry·Stephanie L AngioneAnubhav Tripathi
Mar 6, 2012·Analytical Chemistry·Yingjie YuMichael G Roper
Dec 15, 2012·ACS Nano·Matthew D BlankschienMichael S Wong
May 14, 2011·Advanced Materials·Luc GervaisEmmanuel Delamarche
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Jul 31, 2013·Lab on a Chip·Muhammed S BoybayCarolyn L Ren
Aug 15, 2018·IET Nanobiotechnology·Min-Sheng Hung, Chih-Pin Chen
Apr 2, 2014·Biomedical Optics Express·Eric W Hall, Gregory W Faris
Jan 1, 2014·Journal of the Electrochemical Society·R PrakashK V I S Kaler
Apr 17, 2010·Chemical Reviews·Chunsun Zhang, Da Xing
Jan 21, 2017·Analytical Chemistry·Nicholas M AdamsFrederick R Haselton

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