Nanoelectrode ensembles as recognition platform for electrochemical immunosensors

Biosensors & Bioelectronics
S Pozzi MucelliP Ugo

Abstract

In this study we demonstrate the possibility to prepare highly sensitive nanostructured electrochemical immunosensors by immobilizing biorecognition elements on nanoelectrode ensembles (NEEs) prepared in track-etch polycarbonate membranes. The gold nanodisk electrodes act as electrochemical transducers while the surrounding polycarbonate binds the antibody-based biorecognition layer. The interaction between target protein and antibody is detected by suitable secondary antibodies labelled with a redox enzyme. A redox mediator, added to the sample solution, shuttles electrons from the nanoelectrodes to the biorecognition layer, so generating an electrocatalytic signal. This allows one to fully exploit the highly improved signal-to-background current ratio, typical of NEEs. In particular, the receptor protein HER2 was studied as the target analyte. HER2 detection allows the identification of breast cancer that can be treated with the monoclonal antibody trastuzumab. NEEs were functionalized with trastuzumab which interacts specifically with HER2. The biorecognition process was completed by adding a primary antibody and a secondary antibody labelled with horseradish peroxidase. Hydrogen peroxide was added to modulate the label elec...Continue Reading

References

Dec 31, 2002·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Paolo UgoFrancesca Vezzà
Jun 10, 2005·Nano Letters·Melissa A Lapierre-DevlinShana O Kelley
Aug 11, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Victor C RuckerRenée Shediac
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Citations

Nov 29, 2012·Analytical and Bioanalytical Chemistry·Michael Ongaro, Paolo Ugo
Apr 27, 2011·ACS Applied Materials & Interfaces·Jonathan C ClaussenD Marshall Porterfield
Dec 3, 2014·Protein Expression and Purification·Alexandre ChamasGotthard Kunze
Jan 15, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Joanne I Yeh, Haibin Shi
Aug 18, 2012·Biosensors & Bioelectronics·Morena SilvestriniPaolo Ugo
Aug 12, 2015·Bioelectrochemistry·Andrea RavalliGiovanna Marrazza
Aug 13, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Iulia DiaconuRobert Săndulescu
Jan 4, 2017·Sensors·Najmeh KarimianPaolo Ugo
Sep 8, 2011·Journal of Environmental Monitoring : JEM·Chinnasamy ThiruppathirajaMuthukaruppan Alagar
Dec 11, 2019·Talanta·Maria FreitasCristina Delerue-Matos

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