Nanofiber adsorbents for high productivity continuous downstream processing

Journal of Biotechnology
Oliver HardickDaniel G Bracewell

Abstract

An ever increasing focus is being placed on the manufacturing costs of biotherapeutics. The drive towards continuous processing offers one opportunity to address these costs through the advantages it offers. Continuous operation presents opportunities for real-time process monitoring and automated control with potential benefits including predictable product specification, reduced labour costs, and integration with other continuous processes. Specifically to chromatographic operations continuous processing presents an opportunity to use expensive media more efficiently while reducing their size and therefore cost. Here for the first time we show how a new adsorbent material (cellulosic nanofibers) having advantageous convective mass transfer properties can be combined with a high frequency simulated moving bed (SMB) design to provide superior productivity in a simple bioseparation. Electrospun polymeric nanofiber adsorbents offer an alternative ligand support surface for bioseparations. Their non-woven fiber structure with diameters in the sub-micron range creates a remarkably high surface area material that allows for rapid convective flow operations. A proof of concept study demonstrated the performance of an anion exchange n...Continue Reading

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Citations

Apr 24, 2016·Journal of Chromatography. a·Jan SchwellenbachJochen Strube
Aug 30, 2016·Computational and Structural Biotechnology Journal·Renaud JacquemartJim Stout
Jul 31, 2016·Biotechnology and Bioengineering·Sarah A JohnsonKurt A Brorson
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Mar 6, 2019·BioMed Research International·Sherif Ashraf FahmyMayyada M H El Sayed
Mar 19, 2019·Biotechnology and Bioengineering·Jordan TurnbullDaniel G Bracewell
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Aug 11, 2021·Journal of Chromatography. a·Johannes WinderlJürgen Hubbuch
May 17, 2017·Industrial & Engineering Chemistry Research·Kerianne M DoboszJessica D Schiffman

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