Nanofiber-mediated microRNA delivery to enhance differentiation and maturation of oligodendroglial precursor cells

Journal of Controlled Release : Official Journal of the Controlled Release Society
Hua Jia DiaoSing Yian Chew

Abstract

Remyelination in the central nervous system (CNS) is critical in the treatment of many neural pathological conditions. Unfortunately, the ability to direct and enhance oligodendrocyte (OL) differentiation and maturation remains limited. It is known that microenvironmental signals, such as substrate topography and biochemical signaling, regulate cell fate commitment. Therefore, in this study, we developed a nanofiber-mediated microRNA (miR) delivery method to control oligodendroglial precursor cell (OPC) differentiation through a combination of fiber topography and gene silencing. Using poly(ε-caprolactone) nanofibers, efficient knockdown of OL differentiation inhibitory regulators were achieved by either nanofiber alone (20-40%, p<0.05) or the synergistic integration with miR-219 and miR-338 (up to 60%, p<0.05). As compared to two-dimensional culture, nanofiber topography enhanced OPC differentiation by inducing 2-fold increase in RIP(+) cells (p<0.01) while the presence of miRs further enhanced the result to 3-fold (p<0.001). In addition, nanofiber-mediated delivery of miR-219 and miR-338 promoted OL maturation by increasing the number of MBP(+) cells significantly (p<0.01). Taken together, the results demonstrate the efficacy...Continue Reading

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Citations

May 20, 2015·Advanced Drug Delivery Reviews·Lan Huong NguyenSing Yian Chew
Mar 22, 2016·Expert Opinion on Biological Therapy·Ahad MokhtarzadehMohammad Ramezani
Jan 28, 2016·Regenerative Biomaterials·Liliana R Pires, Ana P Pêgo
Jun 21, 2015·Brain Research·Sarah MoyonPatrizia Casaccia
Dec 16, 2016·Advanced Healthcare Materials·Leo L Wang, Jason A Burdick
Oct 28, 2019·Journal of Biomedical Materials Research. Part a·Aylin TahmasebiAbbas S Moghadam
Sep 12, 2020·Journal of Molecular Neuroscience : MN·Xiaojing YuanQiang Ao
May 26, 2017·Neuromolecular Medicine·I Osorio-QuerejetaD Otaegui
Feb 18, 2017·Journal of Materials Science. Materials in Medicine·Antonio MerolliJoachim Kohn
Jul 28, 2020·Cells·Conor P Duffy, Claire E McCoy
Jul 17, 2020·Journal of Tissue Engineering·Junquan LinSing Yian Chew
Oct 27, 2017·Advanced Healthcare Materials·Caroline M CurtinFergal J O'Brien
Mar 12, 2020·Frontiers in Bioengineering and Biotechnology·Spase Stojanov, Aleš Berlec
Dec 20, 2019·Advanced Healthcare Materials·Junquan LinSing Yian Chew
Dec 4, 2020·Journal of Drug Targeting·Panoraia I SiafakaNeslihan Üstündağ Okur
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Junquan LinSing Yian Chew

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