Nanofiber-mediated release of retinoic acid and brain-derived neurotrophic factor for enhanced neuronal differentiation of neural progenitor cells

Drug Delivery and Translational Research
Wei Ching LowSing Yian Chew

Abstract

The treatment of an injured central nervous system using stem-cell-based regenerative medicine still faces considerable hurdles that need to be overcome. Chief among which is the lack of efficient strategies to generate functional neurons from stem cells. The sustained delivery of biochemical cues and synergistic topographical signaling from electrospun nanofibrous scaffolds may be a potential strategy to enhance neuronal differentiation of stem cells for therapeutic purposes. In this study, retinoic acid (RA) and brain-derived neurotrophic factor (BDNF) were encapsulated into a copolymer of ε-caprolactone and ethyl ethylene phosphate to form a multifunctional, electrospun nanofibrous scaffold. Sustained release of RA and BDNF was achieved for at least 7 and 14 days, respectively. Despite lower cumulative release of drugs as compared to bolus delivery to plain nanofibers (at least 2× and 50× lower for RA and BDNF, respectively), nanofiber-mediated delivery of RA and/or BDNF resulted in similar capacity for neuronal differentiation of mouse neural progenitor cells (NPCs). In addition, nanofiber topography significantly increased neuronal differentiation (with BDNF, 47.4% Map2(+) cells on 2D vs. 53.4 to 56.5% on nanofibers, p < 0...Continue Reading

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Citations

Nov 14, 2013·Archives of Pharmacal Research·Hyun-Ji ParkSeung-Woo Cho
Aug 15, 2020·Frontiers in Medicine·Lei ZhangHong Jiang
Oct 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Febriyani F R DamanikLorenzo Moroni
Jan 31, 2018·ACS Applied Materials & Interfaces·Chia-Chen HsuMolly M Stevens
Aug 8, 2016·ACS Biomaterials Science & Engineering·Ulla MilbretaSing Yian Chew

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