Nanofibrous membrane based tyrosinase-biosensor for the detection of phenolic compounds

Analytica Chimica Acta
Alessandra ArecchiSaverio Mannino

Abstract

A tyrosinase-modified electrode is described to be used as amperometric biosensor for the detection of phenolic compounds in food. The enzyme has been immobilized by drop-coating on a glassy carbon electrode covered by a polyamidic nanofibrous membrane prepared by electrospinning. With respect to others, the selectivity of the designed tyrosinase-biosensor resulted modified by the presence of the nanostructured coating which seems to affect the permeability of phenols as a function of the pH of the solution and of their dissociation constants. The biosensor exhibits a response time of 16 s, a detection limit of 0.05 microM, and a linearity up to 100 microM (slope: -304 nA microM(-1); intercept: -191 nA, r(2)=0.996, n=19). Among others, it can be successfully used for monitoring in real time the release kinetics of phenols encapsulated in polymeric microcapsules.

References

Jul 6, 2007·Bioelectrochemistry·Yashuang LuRuqin Yu
Jul 30, 2008·Critical Reviews in Food Science and Nutrition·M S Fernandez-PanchonM C Garcia-Parrilla

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Citations

May 9, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Iván E Moreno-CortezRodolfo Cruz-Silva
Apr 9, 2014·ACS Applied Materials & Interfaces·Carolina AcostaRámon Martínez-Máñez
Apr 11, 2012·Biomacromolecules·Christina TangSaad A Khan
Jun 24, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yaping HeJianbin Zheng
Jan 1, 2012·Biosensors·Juliano Elvis OliveiraValtencir Zucolotto
Oct 25, 2014·The Analyst·Xiaoyuan JiSongping Zhang
Jun 3, 2021·International Journal of Molecular Sciences·Alexandra Virginia Bounegru, Constantin Apetrei
Oct 22, 2021·Biotechnology and Bioengineering·Anjum Hamid RatherFaheem A Sheikh

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