Nanoformulation of paclitaxel to enhance cancer therapy

Journal of Biomaterials Applications
Quanrong GuJie Chen

Abstract

Paclitaxel is a microtubule inhibitor causing mitotic arrest and is widely used in cancer chemotherapy. However, its poor water solubility restricts its direct clinical applications. In this article, we report paclitaxel-loaded nanoparticles that are water soluble and that can improve the drug's bio-distribution and therapeutic efficacy. Paclitaxel-loaded nanoparticles were synthesized by using Pluronic copolymers (F-68 and P-123) and surfactant (Span 40) as nanocarrier. The toxicity and cellular uptake of paclitaxel-loaded nanoparticles were evaluated. The paclitaxel-loaded nanoparticles can completely disperse into phosphate buffer saline to produce a clear aqueous suspension. Based on HPLC analysis, the drug-loading rate is 9.0 ± 0.1% while drug encapsulation efficiency is 99.0 ± 1.0%. The cytotoxicity assay was performed using breast cancer MCF-7 and cervical cancer Hela cells. For MCF-7 cells, the half maximal inhibitory concentrations (IC50) of paclitaxel-loaded nanoparticles and paclitaxel are 8.5 ± 0.3 and 14.0 ± 0.7 ng/mL at 48 hours and 3.5 ± 0.4 and 5.2 ± 0.5 ng/mL at 72 hours across several runs. IC50 of paclitaxel-loaded nanoparticles and paclitaxel for Hela cells are 5.0 ± 0.3 and 8.0 ± 0.3 ng/mL at 48 hours and 2...Continue Reading

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Citations

May 16, 2017·Journal of Biomaterials Applications·Weiting GuBeihua Kong
Jul 12, 2017·Breast Cancer Research and Treatment·Heba AlshakerDmitri Pchejetski
Jul 7, 2017·Nano Reviews & Experiments·Swati Gupta, Manish K Gupta
May 5, 2020·Anti-cancer Agents in Medicinal Chemistry·Thoria DiabMohamed Hessien
May 21, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Elizabeth CampbellAnton V Naumov

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Methods Mentioned

BETA
transmission
dynamic light scattering
confocal microscopy
transmission electron microscopy

Software Mentioned

RT
LSM

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