Nanog positively regulates Zfp57 expression in mouse embryonic stem cells

Biochemical and Biophysical Research Communications
Yukari YamaguchiHiroshi Koide

Abstract

To maintain the self-renewal of embryonic stem (ES) cells, several core transcription factors, including Oct3/4, STAT3, and Nanog, regulate the expression of their target genes. Zinc finger protein 57 (Zfp57) is specifically expressed in self-renewing ES cells and its expression level is reduced upon ES cell differentiation, suggesting that expression of this transcription factor is regulated by core transcription factors. In the present study, we investigated whether Zfp57 expression is regulated by Nanog. Nanog overexpression resulted in the upregulation of Zfp57. On the other hand, knockdown of Nanog reduced the expression level of Zfp57. In addition, we identified the Nanog-responsive region in the promoter of the Zfp57 gene. These results suggest that Nanog is an upstream regulator of Zfp57. Moreover, Nanog overexpression promoted the growth of ES cells in soft agar and this was suppressed by Zfp57 knockdown, suggesting that the Nanog/Zfp57 pathway plays a central role in anchorage-independent growth of ES cells. Interestingly, NANOG overexpression also led to the upregulation of ZFP57 in two human tumor cell lines. Taken together, our results suggest that Nanog positively regulates Zfp57 expression in multiple types of ce...Continue Reading

References

Jul 5, 2015·Current Allergy and Asthma Reports·Saman SabounchiKari Nadeau
May 15, 2015·Current Opinion in Genetics & Development·Maxim Vc Greenberg, Déborah Bourc'his

Citations

Dec 31, 2003·Biochemical and Biophysical Research Communications·Urara Yoshida-KoideHiroshi Koide
Apr 23, 2005·Biochemical and Biophysical Research Communications·Tadayuki AkagiTakashi Yokota
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Mar 17, 2006·Biochemical and Biophysical Research Communications·Dan PiestunDavid Givol
Jun 13, 2006·Nature·Natalia IvanovaIhor R Lemischka
Jan 12, 2007·Development·Hitoshi Niwa
Oct 16, 2008·Developmental Cell·Xiajun LiAnne C Ferguson-Smith
Jan 1, 2014·International Journal of Cancer. Journal International Du Cancer·Luis E Iv Santaliz-RuizQuintin Pan

Related Concepts

Tumor Cells, Uncertain Whether Benign or Malignant
Biochemical Pathway
SLC22A3 gene
expression vector
IGF2 gene
Zfp-57 protein, human
Promega
Bos taurus
Polymerase Chain Reaction Analysis
Promoter

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.