Abstract
How the pluripotency of stem cells is maintained and the role of transcription factors in this maintenance remain major questions. In the present study, in order to clarify the mechanism underlying the pluripotency of stem cells for the advancement of regenerative medicine, we examined the effect of forced Nanog expression in mesenchymal cells, with a particular focus on osteogenic differentiation. The human mesenchymal stromal cells (hMSCs) or mouse mesenchymal cell line C3H10T1/2 cells were transduced with the Nanog gene or control green fluorescent protein (GFP) gene by using retrovirus vectors. Short-term, forced Nanog gene expression had few effects on the terminal osteogenic differentiation of either hMSCs or C3H10T1/2 cells. To determine its long-term effects, we established C3H10T1/2 cells expressing Nanog constitutively. Constitutive Nanog expression strongly induced osteogenic differentiation of C3H10T1/2 cells. In regard to cell proliferation, constitutive Nanog expression only repressed the proliferation of the cells treated with rhBMP-2. Moreover, Nanog also had the potential to promote the proliferation of C3H10T1/2 cells in the absence of rhBMP-2. Constitutive Nanog expression enhanced phosphorylation of Smad1/5/...Continue Reading
References
Jun 4, 2002·Nature Biotechnology·Janne L SimonsenMoustapha Kassem
Jun 6, 2003·Cell·Kaoru MitsuiShinya Yamanaka
Jun 6, 2003·Cell·Ian ChambersAustin Smith
Jun 5, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Toru OgasawaraHiroshi Kawaguchi
Jul 16, 2004·Molecular and Cellular Biology·Toru OgasawaraHiroto Okayama
Jan 14, 2005·Molecular Biology of the Cell·Masanori TeraiTohru Kiyono
Jun 29, 2005·Stem Cells·Louise HyslopMajlinda Lako
Jul 26, 2005·Nature Medicine·Takako KogaHiroshi Takayanagi
Nov 1, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tamara A Franz-OdendaalP Eckhard Witten
Nov 2, 2005·Biochemical and Biophysical Research Communications·Jingyu ZhangJianwu Dai
Dec 13, 2005·Experimental Hematology·Masayoshi KobuneYoshiro Niitsu
Mar 17, 2006·Biochemical and Biophysical Research Communications·Dan PiestunDavid Givol
May 9, 2006·Bone·Shousaku ItohKatsuhiko Ishihara
Jun 3, 2006·Developmental Cell·Monte M WinslowGerald R Crabtree
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Atsushi SuzukiJuan Carlos Izpisúa Belmonte
Nov 28, 2007·Biochemical and Biophysical Research Communications·Bose S KochupurakkalDavid Givol
Jan 12, 2008·Experimental Cell Research·Masahiro J GoHajime Ohgushi
Apr 25, 2008·Biomaterials·Gert J MeijerClemens A van Blitterswijk
May 15, 2008·Developmental Cell·Shinsuke OhbaUng-Il Chung
Jan 14, 2009·The Journal of Cell Biology·Xin ZhangMajlinda Lako
Dec 5, 2009·Journal of the American College of Cardiology·Joshua M HareWarren Sherman
Jan 9, 2010·Stem Cell Reviews and Reports·Una RiekstinaJanis Ancans
Jun 18, 2010·The Journal of Biological Chemistry·Olivia FromiguéPierre J Marie
Dec 7, 2010·The Journal of Biological Chemistry·Stefano ZanottiErnesto Canalis
Citations
Nov 30, 2012·Journal of Cellular Biochemistry·Yongchang Yao, Yingjun Wang
Jul 4, 2015·Stem Cells and Development·Adam NowakowskiBarbara Lukomska
May 9, 2015·The International Journal of Biochemistry & Cell Biology·Elisabetta LambertiniRoberta Piva
Jun 9, 2015·Cellular Reprogramming·Tadahito SaitoToru Ogasawara
Mar 1, 2014·Tissue Engineering. Part a·Concetta FerrettiMonica Mattioli-Belmonte
Mar 29, 2019·Journal of Cellular Physiology·Kongzu HuZongsheng Yin
Jan 30, 2018·Journal of Cellular Biochemistry·Xiaohua LiuJinyong Luo
Dec 31, 2019·Oxidative Medicine and Cellular Longevity·Feilin LiuJinyu Liu
Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Siddharth ShanbhagKamal Mustafa
Jul 3, 2021·Developmental Cell·Yan GongZhipeng Zou
Nov 16, 2021·Stem Cell Research & Therapy·Kai TieLiaobin Chen