Nanogold bioconjugates for direct and sensitive multiplexed immunosensing

Biosensors & Bioelectronics
P DoboszA Maquieira

Abstract

The use of nanogold bioconjugates for direct detection of the antibody-antigen immunoreaction is addressed. The integration of gold nanoparticles tracers as signal generators in microarray immunosensing and compact disc detection technique show important advantages to reach sensitive, selective, high throughput, reliable and cost-effective assays. For that, a thorough study of the performances of the size of spherical nanogold particles and coating density was developed. The size of the nanoparticle determines the optimal antibody dilution, being the smaller particles the best performing ones. Enhancement effect of lower size is also studied. The gold labeling method do not affects the recognition capability of the labeled proteins. As a proof of concept, the nanoconjugates were used for the simultaneous and direct determination of small molecules. Employing nanogold bioconjugates as recognition labels resulted in robust and reliable assays, reaching a sensitivity of 0.03 and 1.3μg/L for sulfasalazine and atrazine, respectively. This shows that the use of nanogold bioconjugates for direct immunosensing is very competitive, achieving highly sensitive and reproducible assays (RSD<10%). This approach would simultaneously determine...Continue Reading

References

Sep 8, 2000·Science·T A TatonR L Letsinger
Mar 28, 2007·Analytica Chimica Acta·Nuria Pastor-NavarroRosa Puchades
Nov 3, 2011·Bioconjugate Chemistry·Jesús Tamarit-LópezAngel Maquieira
Feb 25, 2014·Expert Opinion on Drug Delivery·Roberto Cao-Milán, Luis M Liz-Marzán
Apr 3, 2014·The Analyst·Thiruppathiraja ChinnasamyHelene Andersson Svahn

❮ Previous
Next ❯

Citations

Sep 22, 2015·Analytical Chemistry·Paulina DoboszÁngel Maquieira
Feb 29, 2016·Analytical and Bioanalytical Chemistry·Sergi MoraisÁngel Maquieira
Nov 30, 2019·Chemistry, an Asian Journal·Wanshan LiuKun Qian
Jan 13, 2017·Analytical and Bioanalytical Chemistry·Ahmed Ali BadranÁngel Maquieira
Aug 7, 2020·Biosensors & Bioelectronics·Salvador MasÁngel Maquieira

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.