Nanomaterial-amplified "signal off/on" electrogenerated chemiluminescence aptasensors for the detection of thrombin

Biosensors & Bioelectronics
Yan LiChengxiao Zhang

Abstract

Two electrogenerated chemiluminescence (ECL) aptasensors for the detection of thrombin were developed using the thrombin binding aptamer (TBA) taken as a molecular recognition element and nanomaterial as a carrier of the ECL capture/signal probe. In the "signal off" aptasensor, the thiolated capture probe (ss-DNA, 12-mer) was self-assembled on the gold nanoparticles (GNPs) which were self-assembled on the surface of gold electrode, and hybridized with six-base segment of the ss-DNA sequence (Tgt-aptamer, 21-mer) containing TBA-I (ss-DNA, 15-mer) tagged with ruthenium complex, producing a high ECL intensity. Introduction of the analyte thrombin triggered the dissociation of the Tgt-aptamer tagged with ruthenium complex from the aptasensors, led to significantly decrease in ECL intensity. The decreased ECL intensity was in proportion to the concentration of thrombin in a range from 2.7×10(-12) to 2.7×10(-9) M with a detection limit of 8×10(-13) M. In the "signal on" aptasensor, the thiolated TBA-I was self-assembled on the gold electrode for capturing thrombin onto the electrode and then the TBA-II (ss-DNA, 29-mer) labeled with single-walled carbon-nanotubes (SWNT)-ECL tag was bound with epitope of thrombin, producing a high ECL ...Continue Reading

Citations

Apr 29, 2011·Analytical Chemistry·Anton B IliukW Andy Tao
Dec 14, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yeqian JinJilin Yan
Aug 15, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shuyu ZhangHui Wei
Apr 1, 2014·IEEE Transactions on Biomedical Circuits and Systems·Scott MacKayJie Chen
Jun 28, 2011·Analytical Biochemistry·Xiaodong CaoSongqin Liu
Jul 9, 2014·Analytical and Bioanalytical Chemistry·Paolo BertoncelloLynn Dennany
Jan 4, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaofei WangChengxiao Zhang
Aug 2, 2017·Chemical Communications : Chem Comm·Yasamin Nasiri Khonsari, Shiguo Sun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.