Nanometric protein arrays on protein-resistant monolayers on silicon surfaces

Journal of the American Chemical Society
Jianhua GuChengzhi Cai

Abstract

We present a novel approach for preparation of nanometric protein arrays, based on binding of avidin molecules to nanotemplates generated by conductive AFM lithography on robust oligo(ethylene glycol)-terminated monolayers on silicon (111) surfaces that are protein-resistant. We showed that only biotinated-BSA but not the native BSA bind to the avidin arrays and that the resulting arrays of biotinated BSA could bind avidin to form protein dots with a feature size of approximately 30 nm. This result demonstrates that the avidin array may serve as templates for preparation of nanoarrays of a wide variety of biotin-tagged proteins for studying their interactions with other protein molecules at nanoscale.

References

Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·D L WilsonD L Kaplan
Feb 9, 2002·Science·Ki-Bum LeeMilan Mrksich
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gang-Yu Liu, Nabil A Amro
May 9, 2002·Chemical Reviews·Jillian M Buriak
May 8, 2003·Journal of the American Chemical Society·Ki-Bum LeeChad A Mirkin
Jun 12, 2003·Journal of the American Chemical Society·Gunjan AgarwalMorley O Stone
Nov 13, 2003·Chemical Reviews·Stephan KrämerChristopher B Gorman
Jan 30, 2004·Journal of the American Chemical Society·Rina Y P LueShao Q Yao

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Citations

Dec 15, 2012·Analytical and Bioanalytical Chemistry·Johnpeter N NgunjiriJayne C Garno
Jan 1, 2007·Nanoscale Research Letters·Paula M MendesJon A Preece
Nov 3, 2010·Journal of the American Chemical Society·Guoting QinChengzhi Cai
Aug 2, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Cancan LiX X Zhu
May 8, 2010·Chemical Communications : Chem Comm·Guoting Qin, Chengzhi Cai
Apr 28, 2011·Chemical Communications : Chem Comm·Debjyoti BandyopadhyayYan-Yeung Luk
Oct 16, 2007·Drug Discovery Today·Christer Wingren, Carl A K Borrebaeck
Apr 17, 2007·Trends in Biotechnology·Olli H LaitinenMarkku S Kulomaa
Jul 9, 2009·Journal of Biomedical Materials Research. Part a·Ray C Schmidt, Kevin E Healy
Jan 21, 2016·Macromolecular Rapid Communications·Lei Shen, Jintao Zhu
Jan 31, 2014·ACS Nano·Eduardo Antonio Della PiaKaren Laurence Martinez
Dec 29, 2006·Small·Jérôme PlainAlain M Jonas
Nov 26, 2008·Angewandte Chemie·Pascal JonkheijmHerbert Waldmann
Jul 8, 2009·Angewandte Chemie·Nirmalya BallavMichael Zharnikov
Aug 2, 2006·Molecular BioSystems·Mahesh UttamchandaniShao Q Yao
Jul 5, 2019·Chemical Communications : Chem Comm·Li QuanTianfu Wu
Apr 26, 2013·Beilstein Journal of Nanotechnology·Thomas BaumgärtelHarald Graaf
Jul 14, 2011·Chemistry, an Asian Journal·Yan Li, Chengzhi Cai
Jul 12, 2018·Scientific Reports·Benjamin LeuschelOlivier Soppera
May 15, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Nirmalya BallavMichael Zharnikov
Apr 14, 2005·Chemical Reviews·Nathaniel L Rosi, Chad A Mirkin
Jul 24, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·B GaripcanJ E Spanier
Jan 29, 2009·Journal of the American Chemical Society·Shahrul A Alang AhmadJason Micklefield
Apr 10, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Aidan G YoungDavid P Green
Nov 8, 2007·Journal of the American Chemical Society·Nicholas P ReynoldsC Neil Hunter
Jul 30, 2009·The Journal of Organic Chemistry·Preeti SejwalYan-Yeung Luk
Aug 25, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Maricel MarquezBrian P Grady
Apr 2, 2010·ACS Applied Materials & Interfaces·Jie-Ren Li, Jayne C Garno
Aug 27, 2013·ACS Nano·Ehtsham Ul-HaqNicholas H Williams
Jul 21, 2006·The Journal of Physical Chemistry. B·D O H TeareJ P S Badyal
May 26, 2006·The Journal of Physical Chemistry. B·Menglong YangBailin Zhang

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