Nanoparticle Binding to Urokinase Receptor on Cancer Cell Surface Triggers Nanoparticle Disintegration and Cargo Release

Theranostics
Shijie LiMingdong Huang

Abstract

Cancer cell expresses abundant surface receptors. These receptors are important targets for cancer treatment and imaging applications. Our goal here is to develop nanoparticles with cargo loading and tumor targeting capability. Methods: A peptide targeting at cancer cell surface receptor (urokinase receptor, uPAR) was expressed in fusion with albumin (diameter of ~7 nm), and the fusion protein was assembled into nanoparticles with diameter of 40 nm, either in the presence or absence of cargo molecules, by a novel preparation method. An important feature of this method is that the nanoparticles were stabilized by hydrophobic interaction of the fusion protein and no covalent linking agent was used in the preparation. The stability, the cargo release, in vitro and in vivo properties of such formed nanoparticles were characterized by transmission electron microscopy, dynamic light scattering, gel shift assay, laser scanning confocal microscopy and 3D fluorescent molecular tomography. Results: The nanoparticles were stable for more than two weeks in aqueous buffer, even in the buffer containing 10% fetal bovine serum. Interestingly, in the presence of urokinase receptor, the uPAR-targeting nanoparticle disintegrated into 7.5 nm frag...Continue Reading

Citations

Oct 4, 2020·IET Nanobiotechnology·Hasnat Tariq, Syed Ali Imran Bokhari
Mar 11, 2020·Experimental & Molecular Medicine·Haili LinPeng Xu
Apr 14, 2020·Nanotechnology, Science and Applications·Nasrul WathoniMuchtaridi Muchtaridi
Jan 25, 2021·Drug Discovery Today·Cai YuanMingdong Huang
Oct 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Salvador GomezZhiwei Hu
Mar 27, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Namdev DhasTejal Mehta
Apr 25, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Libin JiangMingdong Huang

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Methods Mentioned

BETA
dynamic light scattering
ELISA
fluorescence imaging
xenograft
electrophoresis
Protein Assay

Software Mentioned

TrueQuant

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