Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella

Materials
Sumeyra SavasZeynep Altintas

Abstract

Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL-1. The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002⁻200 µM using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection ...Continue Reading

References

Sep 1, 2009·Biosensors & Bioelectronics·Susana LiébanaMaría Isabel Pividori
Dec 10, 2015·Biosensors & Bioelectronics·Noor Azlina MasdorIbtisam E Tothill
Dec 24, 2015·Talanta·Maria PawulaIbtisam E Tothill
Feb 8, 2016·Biosensors & Bioelectronics·Gönül Yenilmez ÇiftçiYıldız Uludağ
May 4, 2016·Applied Microbiology and Biotechnology·Airis Maria Araújo MeloCarlúcio R Alves
Sep 4, 2016·Talanta·Yıldız UludagZeynep Altintas
Oct 28, 2016·Biotechnology Journal·Laura SutarlieXiaodi Su
Oct 11, 2017·Biosensors & Bioelectronics·Zeynep AltintasYildiz Uludag

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Citations

Dec 28, 2019·Materials·Bhargav D Mansuriya, Zeynep Altintas
Dec 8, 2019·Sensors·Muqsit Pirzada, Zeynep Altintas
Nov 30, 2019·Biomedical Microdevices·Yeongseok JangJonghyun Oh
May 7, 2020·Frontiers in Chemistry·Zhengdi WangMin Wei
Feb 23, 2020·Sensors·Bhargav D Mansuriya, Zeynep Altintas
Aug 11, 2020·Biosensors & Bioelectronics·Muqsit PirzadaZeynep Altintas
Nov 12, 2020·International Journal of Molecular Sciences·Mahmuda AkterIhn Han
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yafang ShenYanbin Li

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Methods Mentioned

BETA
biosensor
biosensors
surface plasmon resonance
Chip
biosensing
chips
AFM
Assay
sandwich assay
DNA

Software Mentioned

MiCont TM
MiCont

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