Nanoparticle induced barrier function assessment at liquid-liquid and air-liquid interface in novel human lung epithelia cell lines

Toxicology Research
Lars LeibrockAndreas Luch

Abstract

Inhalation is the most relevant entry point for nanoparticles (NPs) into the human body. To date, toxicity testing of nanomaterials in respect to oral, dermal and inhalative application is mainly based on animal experiments. The development of alternative test methods is the subject of current research. In vitro models can help to investigate mechanistic aspects, as e.g. cellular uptake or genotoxicity and might help to reduce in vivo testing. Lung cell lines are proper in vitro tools to assess NP toxicity. In respect to this, various cell models have been developed during the recent years, but often lack in a proper intact barrier function. However, besides other important in vivo criteria which are still missing like e.g. circulation, this is one basic prerequisite to come closer to the in vivo situation in certain mechanistic aspects such as particle translocation which is an important task for risk assessment of nanomaterials. Novel developed in vitro models may help to investigate the translocation of nanomaterials from the lung. We investigated the barrier function of the recently developed human lung cell lines CI-hAELVi and CI-huAEC. The cells were further exposed to CeO2 NPs and ZnO NPs, and their suitability as in vit...Continue Reading

References

Nov 1, 1973·Journal of the National Cancer Institute·D J GiardW P Parks
Nov 21, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H L WintonC Robinson
Jan 11, 2005·American Journal of Respiratory Cell and Molecular Biology·Barbara M Rothen-RutishauserPeter Gehr
Jul 9, 2005·Environmental Health Perspectives·Günter OberdörsterJan Oberdörster
Jul 16, 2005·Inhalation Toxicology·James S BrownLester D Grant
Jun 16, 2006·Inhalation Toxicology·S TakenakaH Schulz
Dec 23, 2008·Particle and Fibre Toxicology·Béatrice L'azouCéline Ohayon-Courtès
Mar 9, 2010·Particle and Fibre Toxicology·Marianne Geiser, Wolfgang G Kreyling
Apr 3, 2010·Journal of Nanoscience and Nanotechnology·In-Sun KimSoo-Jin Choi
Apr 7, 2010·Chemical Communications : Chem Comm·Talib PirmohamedWilliam T Self
Nov 9, 2010·Nature Biotechnology·Hak Soo ChoiAkira Tsuda
Feb 25, 2011·Particle and Fibre Toxicology·Alexandra KrollJürgen Schnekenburger
Aug 4, 2012·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Carsten SchlehWolfgang G Kreyling
Feb 5, 2013·International Journal of Molecular Sciences·Laura De MarziAnna Poma
Sep 10, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Isabelle GeorgeArmelle Baeza-Squiban
Dec 3, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C Frieke KuperIngeborg M Kooter
Dec 3, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Katrien LuytsPeter H M Hoet
Jan 13, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Xuefang JingPeter S Thorne
Sep 24, 2015·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Nanna B HartmannJuan M Riego Sintes
Nov 26, 2015·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Yasuo MorimotoTakeshi Sasaki
Mar 6, 2016·Journal of Nanobiotechnology·Martin WiemannRobert Landsiedel
Apr 8, 2016·Physica Medica : PM : an International Journal Devoted to the Applications of Physics to Medicine and Biology : Official Journal of the Italian Association of Biomedical Physics (AIFB)·Zi OuyangWilfred Ngwa
Apr 14, 2016·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·C R SvenssonJ Rissler
Oct 30, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Ragini SinghSanjay Singh
May 2, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C BarraudF Sichel
Jun 6, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ajay Vikram SinghMetin Sitti

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Citations

Dec 30, 2020·ACS Applied Materials & Interfaces·Ajay Vikram SinghAndreas Luch
Jul 23, 2021·Advanced Healthcare Materials·Ajay Vikram SinghByung-Wook Park
Jul 14, 2021·Advanced Drug Delivery Reviews·Mohammed Ali SeloCarsten Ehrhardt

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Methods Mentioned

BETA
fluorescence imaging
FCS
transmission
X-ray
chip

Software Mentioned

EpiAlveolar
NTA
Origin

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