Nanoparticle Wettability Influences Nanoparticle-Phospholipid Interactions

Langmuir : the ACS Journal of Surfaces and Colloids
Nagarjun V KonduruRamon M Molina

Abstract

We explored the influence of nanoparticle (NP) surface charge and hydrophobicity on NP-biomolecule interactions by measuring the composition of adsorbed phospholipids on four NPs, namely, positively charged CeO2 and ZnO and negatively charged BaSO4 and silica-coated CeO2, after exposure to bronchoalveolar lavage fluid (BALf) obtained from rats, and to a mixture of neutral dipalmitoyl phosphatidylcholine (DPPC) and negatively charged dipalmitoyl phosphatidic acid (DPPA). The resulting NP-lipid interactions were examined by cryogenic transmission electron microscopy (cryo-TEM) and atomic force microscopy (AFM). Our data show that the amount of adsorbed lipids on NPs after incubation in BALf and the DPPC/DPPA mixture was higher in CeO2 than in the other NPs, qualitatively consistent with their relative hydrophobicity. The relative concentrations of specific adsorbed phospholipids on NP surfaces were different from their relative concentrations in the BALf. Sphingomyelin was not detected in the extracted lipids from the NPs despite its >20% concentration in the BALf. AFM showed that the more hydrophobic CeO2 NPs tended to be located inside lipid vesicles, whereas less hydrophobic BaSO4 NPs appeared to be outside. In addition, cryo-...Continue Reading

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Citations

Jan 30, 2020·Biomaterials Science·Haifeng Fan, Zhiguang Guo
Sep 2, 2020·Advances in Colloid and Interface Science·Feifei WangHongbo Zeng
Jan 21, 2021·International Journal of Molecular Sciences·Paweł KowalczykIda Franiak-Pietryga

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