Nanoparticulate glutathione peroxidase mimics based on selenocystine-pullulan conjugates

Bioconjugate Chemistry
Mamoru HaratakeMorio Nakayama

Abstract

We synthesized nanoparticulate glutathione peroxidase (GPx) mimics in which selenocystine (SeCyst) was conjugated to a hydrophilic linear polysaccharide, pullulan (Pul). The SeCyst ester-conjugated Pul derivatives (SeCyst-Pul) in phosphate buffer (pH 7) were treated with a sonicator to spontaneously form particulate materials. Dynamic light scattering measurements revealed that the SeCyst-Pul conjugates could form particulate materials with diameters between 100 and 300 nm. Distinctive endothermic peaks were observed for the SeCyst-Pul aggregate solutions based on a differential scanning calorimetric analysis. The tryptophan (Trp) fluorescence intensity of SeCyst benzyl ester-tryptophanyl-Pul (SeCyst-Bz-Trp-Pul) mostly decreased in comparison to those of the Trp-Pul (its precursor) and free Trp, which indicates that the Trp residues come close to each other during the aggregation of the conjugates. Formation of SeCyst-Pul aggregates could be induced by the hydrophobic interactions between the SeCyst esters and the amino acid residues on Pul. The GPx-like activity of SeCyst-Bz-Trp-Pul aggregates for the reduction of H2O2 was enhanced nearly 20-fold higher than that of free SeCyst. The double-reciprocal plots of the SeCyst-Bz-Trp...Continue Reading

References

Jan 1, 1985·Methods in Enzymology·B Mannervik
Jan 1, 1974·Biochemistry·Y BursteinH Neurath
Jan 1, 1984·International Journal of Peptide and Protein Research·M Bodanszky
Jun 1, 1983·European Journal of Biochemistry·O EppA Wendel
Dec 21, 1993·Biochemistry·I M Bell, D Hilvert
Nov 1, 1995·Chemical & Pharmaceutical Bulletin·H NogusaK Inoue
Jun 27, 1998·Biochemical and Biophysical Research Communications·J Q LiuJ C Shen
Sep 25, 1998·The Journal of Biological Chemistry·A K Singh, H Shichi
Nov 24, 1999·Free Radical Biology & Medicine·R Brigelius-Flohé
Mar 8, 2000·BioFactors·A Böck
Aug 30, 2000·Lancet·M P Rayman
Oct 12, 2000·Nature·J F Atkins, R F Gesteland
May 9, 2002·Molecular and Cellular Biology·Dolph L Hatfield, Vadim N Gladyshev
May 19, 2004·The Journal of Biological Chemistry·Ye SunGuimin Luo
Jan 24, 2006·Molecular and Cellular Endocrinology·Joël R Drevet
Aug 29, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ji Sun ParkYong Woo Cho
Feb 6, 2007·Nature Structural & Molecular Biology·Tamara L Hendrickson

❮ Previous
Next ❯

Citations

Mar 3, 2011·Metallomics : Integrated Biometal Science·Mamoru HaratakeMorio Nakayama
Sep 14, 2012·Chemical Society Reviews·Zeyuan DongJunqiu Liu
Jan 24, 2015·Bioconjugate Chemistry·Gwénaëlle BonziPaolo Caliceti
Sep 7, 2016·Journal of Theoretical Biology·Ramesh KheirabadiMohammad Reza Housiandokht
Oct 23, 2016·Free Radical Biology & Medicine·Christopher L BiancoJohn P Toscano
Jul 16, 2010·Macromolecular Bioscience·Xin HuangJunqiu Liu
Nov 23, 2013·Organic & Biomolecular Chemistry·Lu MiaoJunqiu Liu
Apr 12, 2016·Chemistry : a European Journal·Evelyn KuahZhiqiang Gao
Apr 21, 2018·Chemical Science·Nicolas ChuardStefan Matile
Apr 10, 2021·APL Bioengineering·Wonmoon SongNathaniel S Hwang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.