Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement

ACS Applied Materials & Interfaces
Ha-Rim SeoDo-Sun Lim

Abstract

Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1+ MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1+ MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres with mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized pillars enhanced cardiomyocyte differentiation in Flk1+ MPCs.

References

Apr 9, 2005·Biomaterials·Evelyn K F YimKam W Leong
Jul 22, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun K YamashitaShin-Ichi Nishikawa
Dec 20, 2008·Biochemical and Biophysical Research Communications·Peishi YanJun K Yamashita
Feb 9, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Manus Jonathan Paul BiggsMatthew J Dalby
May 28, 2010·International Journal of Cancer. Journal International Du Cancer·Bo Mi KuSang Soo Kang
Dec 15, 2010·European Cells & Materials·Chung-Yao YangJ Andrew Yeh
Apr 12, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jin-Woo LeeHun-Kuk Park
May 2, 2012·The Journal of Cell Biology·Deok-Ho KimAndre Levchenko
Oct 11, 2012·PloS One·Anne BlangyGeraldine Pawlak
Nov 28, 2012·Journal of Biomedical Materials Research. Part a·Evon S EreifejPamela J VandeVord
Oct 5, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Marcus S NiepelThomas Groth
Jul 6, 2014·Journal of Clinical and Diagnostic Research : JCDR·Gk ThakralPallavi Vashisht
Dec 4, 2014·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Jessie R WangStephen J Yarwood
Mar 31, 2015·World Journal of Stem Cells·Shima SalmasiAlexander M Seifalian
Nov 18, 2016·Journal of the American Heart Association·Sung Woo ChoSeung-Hyuk Choi

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Citations

Jun 6, 2020·Annual Review of Biomedical Engineering·Erica A CastilloBeth L Pruitt
Mar 25, 2019·Stem Cells International·Rosaria SantoroGiulio Pompilio
Oct 10, 2020·Advanced Biosystems·Seong-Beom HanDong-Hwee Kim
Feb 12, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Priya Mohindra, Tejal A Desai
Mar 17, 2020·Biosensors & Bioelectronics·Jong Min LeeBong Geun Chung
Sep 10, 2019·Colloids and Surfaces. B, Biointerfaces·Jie HuYong Chen

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