Nanoplasmonic mid-infrared biosensor for in vitro protein secondary structure detection

Light, Science & Applications
Dordaneh EtezadiHatice Altug

Abstract

Plasmonic nanoantennas offer new applications in mid-infrared (mid-IR) absorption spectroscopy with ultrasensitive detection of structural signatures of biomolecules, such as proteins, due to their strong resonant near-fields. The amide I fingerprint of a protein contains conformational information that is greatly important for understanding its function in health and disease. Here, we introduce a non-invasive, label-free mid-IR nanoantenna-array sensor for secondary structure identification of nanometer-thin protein layers in aqueous solution by resolving the content of plasmonically enhanced amide I signatures. We successfully detect random coil to cross β-sheet conformational changes associated with α-synuclein protein aggregation, a detrimental process in many neurodegenerative disorders. Notably, our experimental results demonstrate high conformational sensitivity by differentiating subtle secondary-structural variations in a native β-sheet protein monolayer from those of cross β-sheets, which are characteristic of pathological aggregates. Our nanoplasmonic biosensor is a highly promising and versatile tool for in vitro structural analysis of thin protein layers.

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Citations

Nov 8, 2018·The Analyst·Gregory Q Wallace, François Lagugné-Labarthet
May 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natalia WilkoszKamila Sofińska
Apr 3, 2020·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Christoph KratzKarsten Hinrichs
Mar 25, 2018·Advanced Materials·Xiaoxia YangQing Dai
Oct 3, 2018·Light, Science & Applications·Yibo ZhuQiao Lin
Apr 2, 2021·Optics Letters·Romil Audhkhasi, Michelle L Povinelli
May 28, 2021·The Journal of Physical Chemistry. B·Rodrigo Noriega
Jun 28, 2018··Aleksandrs LeitisDaniel Rodrigo

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Methods Mentioned

BETA
biosensing
infrared absorption spectroscopy
biosensors
biosensor
chips
AFM
Chip

Software Mentioned

Lumerical FDTD Solutions

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