Nanopore Sequencing in Blood Diseases: A Wide Range of Opportunities

Frontiers in Genetics
Crescenzio Francesco MinerviniFrancesco Albano

Abstract

The molecular pathogenesis of hematological diseases is often driven by genetic and epigenetic alterations. Next-generation sequencing has considerably increased our genomic knowledge of these disorders becoming ever more widespread in clinical practice. In 2012 Oxford Nanopore Technologies (ONT) released the MinION, the first long-read nanopore-based sequencer, overcoming the main limits of short-reads sequences generation. In the last years, several nanopore sequencing approaches have been performed in various "-omic" sciences; this review focuses on the challenge to introduce ONT devices in the hematological field, showing advantages, disadvantages and future perspectives of this technology in the precision medicine era.

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Citations

May 18, 2020·International Journal of Molecular Sciences·Cosimo CumboFrancesco Albano
Sep 13, 2020·International Journal of Molecular Sciences·Crescenzio Francesco MinerviniFrancesco Albano
May 23, 2020·Cancer Management and Research·Cosimo CumboFrancesco Albano
Jul 17, 2020·Frontiers in Immunology·Richard B KennedyGregory A Poland
May 6, 2020·International Journal of Molecular Sciences·Nicoletta CoccaroFrancesco Albano
Nov 10, 2020·Critical Reviews in Clinical Laboratory Sciences·Claire AndrewsMark Minden
Jan 9, 2021·Frontiers in Physiology·Roberta RussoImmacolata Andolfo
Apr 26, 2021·Seminars in Cancer Biology·Jörg Westermann, Lars Bullinger
Dec 12, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Teng ZhouLiping Wen
Sep 5, 2021·Scientific Reports·Crescenzio Francesco MinerviniFrancesco Albano

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Methods Mentioned

BETA
Polymerase Chain Reaction
PCR
RNA-seq
Genotyping

Software Mentioned

PromethION
ONT
WhatsHap
MinION
GridION

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