Nanoporesize affects complement activation

Journal of Biomedical Materials Research. Part a
Natalia FerrazMarjam Karlsson Ott

Abstract

In the present study, we have shown the vast importance of biomaterial nanotexture when evaluating inflammatory response. For the first time in an in vitro whole blood system, we have proven that a small increase in nanoporesize, specifically 180 nm (from 20 to 200 nm), has a huge effect on the complement system. The study was done using nanoporous aluminiumoxide, a material that previously has been evaluated for potential implant use, showing good biocompatibility. This material can easily be manufactured with different pore sizes making it an excellent candidate to govern specific protein and cellular events at the tissue-material interface. We performed whole blood studies, looking at complement activation after blood contact with two pore size alumina membranes (pore diameters, 20 and 200 nm). The fluid phase was analyzed for complement soluble components, C3a and sC5b-9. In addition, surface adsorbed proteins were eluted and dot blots were performed to detect IgG, IgM, C1q, and C3. All results point to the fact that 200 nm pore size membranes are more complement activating. Significantly, higher values of complement soluble components were found after whole blood contact with 200 nm alumina and all studied proteins adsorbe...Continue Reading

References

Jan 1, 1992·Scandinavian Journal of Immunology·K Nilsson EkdahlU R Nilsson
Aug 1, 1994·Biomaterials·J M CourtneyC D Forbes
Mar 15, 1996·Experimental Cell Research·B Wójciak-StothardC Wilkinson
Jun 5, 1998·Biomaterials·A Curtis, C Wilkinson
Apr 23, 1999·Biomaterials·R G FlemmingP F Nealey
Jul 8, 1999·Biomaterials·T J WebsterR Bizios
Feb 17, 2001·Trends in Biotechnology·A Curtis, C Wilkinson
Mar 30, 2001·Advances in Dental Research·B Kasemo, J Gold
Sep 13, 2001·Journal of Biomedical Materials Research·J WetteröP Tengvall
Jan 17, 2002·Biomaterials·Jonas WetteröPentti Tengvall
Jun 19, 2002·Biomaterials·M J DalbyA S G Curtis
Jun 14, 2003·Medical & Biological Engineering & Computing·R L PriceT J Webster
Jun 18, 2003·Biomaterials·Ann-Sofie AnderssonBengt Kasemo
Aug 5, 2003·Biomaterials·M KarlssonL Di Silvio
Oct 2, 2003·Nature Biotechnology·George M Whitesides
Oct 23, 2003·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Heinrich WienekeRaimund Erbel
Jun 2, 2004·Microscopy Research and Technique·M KarlssonM Boman
Sep 24, 2004·IEEE Transactions on Nanobioscience·A S G CurtisG Aitchison
Aug 15, 2006·Molecular Immunology·Bo NilssonJohn D Lambris
Feb 13, 2007·Nanomedicine : Nanotechnology, Biology, and Medicine·Evelyn K F Yim, Kam W Leong

❮ Previous
Next ❯

Citations

May 24, 2011·Seminars in Immunopathology·Werner Zimmerli, Parham Sendi
Apr 17, 2008·Journal of Materials Science. Materials in Medicine·Natalia FerrazMarjam Karlsson Ott
Jul 24, 2013·Advanced Drug Delivery Reviews·Anne Mari A RokstadBerit L Strand
Jan 15, 2011·International Journal of Biomaterials·Natalia FerrazMarjam Karlsson Ott
May 13, 2008·Journal of Biomedical Materials Research. Part a·Fredrik BexbornKristina Nilsson Ekdahl
Jun 8, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Andrey SokolovTom E Mollnes
Feb 15, 2011·Journal of Biomedical Materials Research. Part a·Anna E EngbergKristina N Ekdahl
Apr 12, 2012·Journal of Biomedical Materials Research. Part a·Andreas HoessAndreas Heilmann
Nov 30, 2013·Journal of Biomedical Materials Research. Part a·Shiuli PujariMarjam Karlsson-Ott
Jul 8, 2009·Journal of Biomaterials Applications·Natalia FerrazMarjam Karlsson Ott
Sep 7, 2018·Journal of Cellular Physiology·Maryam Rahmati, Masoud Mozafari
Mar 17, 2010·Microscopy Research and Technique·Natalia FerrazJaan Hong
Oct 31, 2018·International Journal of Molecular Sciences·Yvonne MödingerAnita Ignatius
Sep 9, 2019·ACS Biomaterials Science & Engineering·Mats HulanderMartin Andersson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Materials Science. Materials in Medicine
Natalia FerrazMarjam Karlsson Ott
Journal of Biomaterials Applications
Natalia FerrazMarjam Karlsson Ott
Journal of Biomedical Materials Research. Part a
Erin E Leary SwanTejal A Desai
Journal of Biomedical Materials Research. Part a
Fredrik BexbornKristina Nilsson Ekdahl
© 2021 Meta ULC. All rights reserved