Nanoporous Semiconductor Electrode Captures the Quantum Dots: Toward Ultrasensitive Signal-On Liposomal Photoelectrochemical Immunoassay

Analytical Chemistry
Tie-Ying XueWei-Wei Zhao

Abstract

Liposomal photoelectrochemical (PEC) bioanalysis has recently emerged and exhibited great potential in sensitive biomolecular detection. Exploration of the facile and efficient route for advanced liposomal PEC bioanalysis is highly appealing. In this work, we report the split-type liposomal PEC immunoassay system consisting of sandwich immunorecognition, CdS quantum dots (QDs)-loaded liposomes (QDLL), and the release and subsequent capture of the QDs by a separated TiO2 nanotubes (NTs) electrode. The system elegantly operated upon the protein binding and lysis treatment of CdS QDLL labels within the 96-well plate, and then the CdS QDs-enabled sensitization of TiO2 NTs electrode. Exemplified by cardiac markers troponin I (cTnI) as target, the proposed system achieved efficient activation of TiO2 NTs electrode and thus the signal generation toward the split-type PEC immunoassay. This work features the first use of QDs for liposomal PEC bioanalysis and is expected to inspire more interests in the design and implementation of numerous QDs-involved liposomal PEC bioanalysis.

References

Feb 3, 2005·Nature Reviews. Drug Discovery·Vladimir P Torchilin
Sep 14, 2010·Chemical Reviews·Anders HagfeldtHenrik Pettersson
Oct 18, 2012·The Analyst·Qingtao Liu, Ben J Boyd
Feb 7, 2013·Analytical Chemistry·Adaikkappan PeriyakaruppanJessica E Koehne
Feb 22, 2013·ACS Applied Materials & Interfaces·Ronit FreemanItamar Willner
Jun 17, 2014·Chemical Reviews·Wei-Wei ZhaoHong-Yuan Chen
Sep 17, 2014·Chemical Society Reviews·Wei-Wei ZhaoHong-Yuan Chen
Dec 3, 2014·Biosensors & Bioelectronics·Satish K TutejaAkash Deep
May 30, 2015·Analytical Chemistry·Wei-Wei ZhaoHong-Yuan Chen
Oct 27, 2015·Biosensors & Bioelectronics·Kihyun KimJeong-Soo Lee
Jun 13, 2017·Advances in Colloid and Interface Science·Federico MazurRona Chandrawati
Sep 21, 2017·Analytical Chemistry·Nan HaoKun Wang
Nov 15, 2017·Analytical Chemistry·Wei-Wei ZhaoHong-Yuan Chen

❮ Previous
Next ❯

Citations

Aug 18, 2020·Critical Reviews in Analytical Chemistry·Guangyan LiuJianping Li
Jun 26, 2020·Frontiers in Bioengineering and Biotechnology·Chaohong ShiJingjing Zhang
May 7, 2020·ACS Applied Materials & Interfaces·Olga A GoryachevaNikolai Gaponik
Dec 22, 2021·Chemical Communications : Chem Comm·Xuechen ZhangWenbo Song

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.