Nanoprecipitation of polymeric nanoparticle micelles based on 2-methacryloyloxyethyl phosphorylcholine (MPC) with 2-(diisopropylamino)ethyl methacrylate (DPA), for intracellular delivery applications

Journal of Materials Science. Materials in Medicine
Jonathan P SalvageAndrew W Lloyd

Abstract

Biodistribution of nanoparticle-based intracellular delivery systems is mediated primarily by particle size and physicochemical properties. As such, overcoming the rapid removal of these by the reticuloendothelial system remains a significant challenge. To date, a number of copolymer nanoparticle systems based on 2-methacryloyloxyethyl phosphorylcholine (MPC) with 2-(diisopropylamino)ethyl methacrylate (DPA), displaying biomimetic and pH responsive properties, have been published, however these have been predominately polymersome based, whilst micelle systems have remained relatively unexplored. This study utilised nanoprecipitation to investigate the effects of solvent and buffer choice upon micelle size and polydispersity, and found using methanol produced monodisperse micelles of circa 70 nm diameter, whilst ethanol produced polydisperse systems with nanoparticles of circa 128 nm diameter. The choice of aqueous buffer, dialysis of the systems, extended storage, and exposure to a wide temperature range (5-70 °C) had no significant effect on micelle size, and the systems were highly resistant to dilution, indicating excellent colloidal stability. Optimisation of the nanoprecipitation process, post precipitation, was investigat...Continue Reading

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Citations

May 10, 2017·The Journal of Pharmacy and Pharmacology·Bahaa AzmyJonathan P Salvage
Jun 21, 2017·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Abdul Khaliq Elzhry ElyafiJonathan P Salvage
Sep 8, 2020·Drug Development and Industrial Pharmacy·Kıvılcım Öztürk-AtarSema Çalış
Aug 10, 2021·Molecular Aspects of Medicine·Alysia CoxEun Ji Chung

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Methods Mentioned

BETA
mechanical filtration
nanoprecipitation
nuclear magnetic resonance
atomic
transmission electron microscopy
AFM
FCS

Software Mentioned

Malvern PCS

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