Nanoscale affinity chip interface for coupling inhibition SPR immunosensor screening with Nano-LC TOF MS

Analytical Chemistry
G R MarchesiniM W F Nielen

Abstract

The on-line nanoscale coupling of a surface plasmon resonance (SPR)-based inhibition biosensor immunoassay (iBIA) for the screening of low molecular weight molecules with nano-liquid-chromatography electrospray ionization time-of-flight mass spectrometry (nano-LC ESI TOF MS) for identification is described. The interface is based on a reusable recovery chip (RC) that contains a nanoscale biosorbent composed of a hydrogel layer modified with antibodies raised against the analyte featuring the unique possibility of performance characterization using the SPR biosensor. Various hydrogel chemistries were evaluated, and the standard Biacore CM5 chip showed the highest capture capacity in combination with affinity-purified polyclonal antibodies. The procedure has four stages: the samples are prepared (1) and screened using a screening chip (SC) in the iBIA (2). Suspected noncompliant samples as being noncompliant are reinjected over the RC, and the analyte is captured at subnanogram level (3). The captured analyte is released, and the eluate is analyzed with nano-LC ESI TOF MS via a loop-type interface (4). The coupling of the technologies proved effective for screening enrofloxacin, a model compound, in incurred chicken muscle sample...Continue Reading

References

May 19, 1998·Trends in Biochemical Sciences·D G Myszka, T A Morton
Mar 17, 1999·Journal of Molecular Recognition : JMR·S HowellR A Badley
Jul 3, 2003·Trends in Biotechnology·Dobrin Nedelkov, Randall W Nelson
Sep 15, 2004·Analytical Chemistry·Jonas Borch, Peter Roepstorff
Apr 30, 2005·Analytical Chemistry·Jens GroteSimone Koenig
Jun 26, 2007·Analytical Chemistry·Dobrin Nedelkov

❮ Previous
Next ❯

Citations

Jul 15, 2011·Analytical and Bioanalytical Chemistry·Jeroen KoolWilfried M A Niessen
Aug 10, 2010·Journal of the American Society for Mass Spectrometry·Mihaela DragusanuMichael Przybylski
Jul 19, 2001·Chemosphere·S D Dyer
Jul 21, 2011·Se pu = Chinese journal of chromatography·Haifang LiJin-Ming Lin
Apr 8, 2010·The Review of Scientific Instruments·Yu LiuWeiqing Xu
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Oct 31, 2009·Journal of Separation Science·María Asensio-RamosSalvatore Fanali
Oct 14, 2009·Journal of Biomolecular Screening·Zhengrong Zhu, John Cuozzo
May 30, 2019·Analytical and Bioanalytical Chemistry·Jinjuan XueHuwei Liu
Apr 10, 2012·Analytica Chimica Acta·Akhtar HayatJean-Louis Marty
Feb 18, 2017·Analytical Chemistry·Sweccha JoshiMichel W F Nielen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosensor Development & Applications

Biosensor development and applications describe recent progress in biosensor development and their applications in the field of antibiotics and metabolic engineering. Discover the latest research on biosensor development and applications in this feed.