Nanoscale analysis of unstained biological specimens in water without radiation damage using high-resolution frequency transmission electric-field system based on FE-SEM

Biochemical and Biophysical Research Communications
Toshihiko Ogura

Abstract

Scanning electron microscopy (SEM) has been widely used to examine biological specimens of bacteria, viruses and proteins. Until now, atmospheric and/or wet biological specimens have been examined using various atmospheric holders or special equipment involving SEM. Unfortunately, they undergo heavy radiation damage by the direct electron beam. In addition, images of unstained biological samples in water yield poor contrast. We recently developed a new analytical technology involving a frequency transmission electric-field (FTE) method based on thermionic SEM. This method is suitable for high-contrast imaging of unstained biological specimens. Our aim was to optimise the method. Here we describe a high-resolution FTE system based on field-emission SEM; it allows for imaging and nanoscale examination of various biological specimens in water without radiation damage. The spatial resolution is 8 nm, which is higher than 41 nm of the existing FTE system. Our new method can be easily utilised for examination of unstained biological specimens including bacteria, viruses and protein complexes. Furthermore, our high-resolution FTE system can be used for diverse liquid samples across a broad range of scientific fields, e.g. nanoparticle...Continue Reading

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Citations

May 24, 2016·Scientific Reports·Christine G GoldingTimothy F Booth
Jul 28, 2016·Stem Cells Translational Medicine·Taisuke FurutaMitsuo Ochi
Nov 17, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Eriko FukudaNaoki Goshima
Jul 10, 2019·Science Advances·Tomoaki IwayamaShinya Murakami
Jul 31, 2019·International Journal of Molecular Sciences·Koki KanehiraMasahiro Yao
Mar 7, 2021·International Journal of Molecular Sciences·Tomoko Okada, Toshihiko Ogura
Mar 20, 2019·Analytical Chemistry·Yukako SengaShinya Honda

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