Nanoscale characterization illustrates the cisplatin-mediated biomechanical changes of B16-F10 melanoma cells

Physical Chemistry Chemical Physics : PCCP
Mei-Lang KungShuchen Hsieh

Abstract

Cells reorganize their membrane biomechanical dynamics in response to environmental stimuli or inhibitors associated with their physiological/pathological processes, and disease therapeutics. To validate the biophysical dynamics during cell exposure to anti-cancer drugs, we investigate the nanoscale biological characterization in melanoma cells undergoing cisplatin treatment. Using atomic force microscopy, we demonstrate that the cellular morphology and membrane ultrastructure are altered after exposure to cisplatin. In contrast to their normal spindle-like shape, cisplatin causes cell deformation rendering cells flat and enlarged, which increases the cell area by 3-4 fold. Additionally, cisplatin decreases the topography height values for both the cytoplasmic and nuclear regions (by 40-80% and 60%, respectively). Furthermore, cisplatin increases the cytoplasmic root mean square roughness by 110-240% in correlation with the drug concentration and attenuates the nuclear RMS by 60%. Moreover, the cellular adhesion force was enhanced, while the Young's modulus elasticity was attenuated by ∼2 and ∼2.3 fold, respectively. F-actin phalloidin staining revealed that cisplatin enlarges the cell size through enhanced stress fiber formati...Continue Reading

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Citations

May 18, 2019·Metallomics : Integrated Biometal Science·Martina RaudenskaMichal Masarik
Apr 3, 2020·International Journal of Oncology·Alice ZamagniChiara Molinari
Aug 24, 2017·Scientific Reports·Chao-Hung ChangChau-Hwang Lee
Oct 30, 2020·International Journal of Molecular Sciences·Chien-Hui WengMing-Hong Tai
Aug 24, 2017·Seminars in Cell & Developmental Biology·S KasasG Dietler

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