Nanoscale-enhanced Ru(bpy)3(2+) electrochemiluminescence labels and related aptamer-based biosensing system

The Analyst
Weiwei GuoErkang Wang

Abstract

A unique multilabeling at a single-site protocol of the Ru(bpy)(3)(2+) electrochemiluminescence (ECL) system is proposed. Nanoparticles (NPs) were used as assembly substrates to enrich ECL co-reactants of Ru(bpy)(3)(2+) to construct nanoscale-enhanced ECL labels. Two different kinds of NP substrates [including semiconductor NPs (CdTe) and noble metal NPs (gold)] capped with 2-(dimethylamino)ethanethiol (DMAET) [a tertiary amine derivative which is believed to be one of the most efficient of co-reactants of the Ru(bpy)(3)(2+) system] were synthesized through a simple one-pot synthesis method in aqueous media. Although both CdTe and gold NPs realized the enrichment of ECL co-reactants, they presented entirely different ECL performances as nanoscale ECL co-reactants of Ru(bpy)(3)(2+). The different effects of these two NPs on the ECL of Ru(bpy)(3)(2+) were studied. DMAET-capped CdTe NPs showed enormous signal amplification of Ru(bpy)(3)(2+) ECL, whereas DMAET-capped gold NPs showed a slight quenching effect of the ECL signal. DMAET-capped CdTe NPs can be considered to be excellent nanoscale ECL labels of the Ru(bpy)(3)(2+) system, as even a NP solution sample of 10(-18) M was still detectable after an electrostatic self-assembly c...Continue Reading

References

Jun 10, 2004·Chemical Reviews·Mark M Richter
Dec 2, 2004·Analytical Chemistry·Eva BaldrichCiara K O'Sullivan
Feb 16, 2006·Journal of the American Chemical Society·Jacob A HansenGreg Collins
Jul 21, 2006·The Journal of Physical Chemistry. B·Sergey K PoznyakNikolai Gaponik

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Citations

Jan 29, 2013·Biosensors & Bioelectronics·Johannes DapràNoemi Rozlosnik
Jul 2, 2010·Chemical Society Reviews·Lianzhe Hu, Guobao Xu
Mar 15, 2011·Luminescence : the Journal of Biological and Chemical Luminescence·Hui Wei, Erkang Wang
Dec 16, 2011·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Jing Li, Erkang Wang
Jan 29, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Huifeng XuGuonan Chen

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