Nanoscale polysaccharide derivative as an AEG-1 siRNA carrier for effective osteosarcoma therapy

International Journal of Nanomedicine
Fen WangLi-Ming Zhang

Abstract

Nanomedicine, which is the application of nanotechnology in medicine to make medical diagnosis and treatment more accurate, has great potential for precision medicine. Despite some improvements in nanomedicine, the lack of efficient and low-toxic vectors remains a major obstacle. The aim of this study was to prepare an efficient and low-toxic vector which could deliver astrocyte elevated gene-1 (AEG-1) small interfering RNA (siRNA; siAEG-1) into osteosarcoma cells effectively and silence the targeted gene both in vitro and in vivo. We prepared a novel polysaccharide derivative by click conjugation of azidized chitosan with propargyl focal point poly (L-lysine) dendrons (PLLD) and subsequent coupling with folic acid (FA; Cs-g-PLLD-FA). We confirmed the complexation of siAEG-1and Cs-g-PLLD or Cs-g-PLLD-FA by gel retardation assay. We examined the cell cytotoxicity, cell uptake, cell proliferation and invasion abilities of Cs-g-PLLD-FA/siAEG-1 in osteosarcoma cells. In osteosarcoma 143B cells tumor-bearing mice models, we established the therapeutic efficacy and safety of Cs-g-PLLD-FA/siAEG-1. Cs-g-PLLD-FA could completely encapsulate siAEG-1 and showed low cytotoxicity in osteosarcoma cells and tumour-bearing mice. The Cs-g-PLLD-...Continue Reading

Citations

Dec 15, 2020·International Journal of Biological Macromolecules·Parisa Maleki DanaBahman Yousefi
Feb 1, 2021·Cancer Treatment and Research Communications·Azam Bozorgi, Leila Sabouri
May 1, 2021·Journal of Clinical Medicine·Shicheng YangSrinivas Sridhar

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Methods Mentioned

BETA
transfection
infrared
nuclear magnetic resonance
electrophoresis
flow cytometry
xenograft
transmission electron microscopy
Fluorescence
flow

Software Mentioned

FV10
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