Nanoscale protein assemblies from a circular permutant of the tobacco mosaic virus

Nano Letters
Michel T DedeoMatthew B Francis

Abstract

The protein coat of the tobacco mosaic virus (TMV) has been explored extensively for the construction of nanoscale architectures. In previous work, we have reported efficient TMV-based light harvesting systems bearing chromophores in a hollow channel of the assembled protein. We have also reported an N-terminal transamination/oximation method that could be used to attach electrodes and catalytic groups to the exterior surface of the rods. To complement these techniques, we report herein a new circular permutant of the TMV capsid protein that repositions the N- and C-termini to the center of the assemblies. This protein can be produced in very high yield through E. coli expression and self-assembles into light harvesting rods that are much like those assembled from the wild-type protein. However, the disks formed from the permutant structure are stable over a significantly wider pH range, greatly improving the practicality of this assembled form for materials applications. The new position of the N-terminus allows functional groups to be installed in the inner pore of the disks, affording geometries reminiscent of natural photosynthetic systems. The permutant also shows the ability to coassemble with regular monomers, allowing t...Continue Reading

References

Apr 5, 1983·Journal of Molecular Biology·D P Goldenberg, T E Creighton
Nov 25, 1982·Nucleic Acids Research·M Gouy, C Gautier
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A BoppR M Hochstrasser
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A Klug
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P J Butler
Aug 17, 1999·Current Opinion in Structural Biology·L Regan
Nov 14, 2000·Current Opinion in Plant Biology·K K Niyogi
Mar 18, 2005·Journal of the American Chemical Society·Tara L SchlickMatthew B Francis
Aug 23, 2005·Angewandte Chemie·Rafael A VegaChad A Mirkin
Oct 20, 2006·Angewandte Chemie·Anouk DirksenPhilip E Dawson
Feb 27, 2007·Journal of the American Chemical Society·Rebekah A MillerMatthew B Francis
Apr 27, 2007·Angewandte Chemie·Patrick G Holder, Matthew B Francis
May 4, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Zhongwei NiuQian Wang
Sep 13, 2007·Chemistry : a European Journal·Masayuki EndoTetsuro Majima
May 13, 2008·The Journal of Physical Chemistry. B·Ying-Zhong MaMatthew B Francis
Aug 8, 2008·Journal of the American Chemical Society·Rebecca A ScheckMatthew B Francis

❮ Previous
Next ❯

Citations

Aug 5, 2011·Accounts of Chemical Research·Leah S Witus, Matthew B Francis
Nov 12, 2010·Journal of the American Chemical Society·Leah S WitusMatthew B Francis
Dec 28, 2011·Nano Letters·Omar Khalil Zahr, Amy Szuchmacher Blum
Nov 17, 2011·Nature Chemical Biology·Nicholas Stephanopoulos, Matthew B Francis
May 13, 2014·Applied Microbiology and Biotechnology·Jeff Glasgow, Danielle Tullman-Ercek
Aug 7, 2010·Current Opinion in Biotechnology·Carissa M Soto, Banahalli R Ratna
Jun 26, 2014·Journal of the American Chemical Society·Allie C ObermeyerMatthew B Francis
Sep 15, 2011·Biotechnology and Bioengineering·Sang-Yup LeeMichael T Harris
Oct 31, 2014·Proteins·Maayan Bonjack, David Avnir
Mar 31, 2015·Virology·James N CulverReza Ghodssi
Jun 27, 2017·Journal of the American Chemical Society·Joel A FinbloomMatthew B Francis
Mar 2, 2016·International Journal of Molecular Sciences·Xiangyang LiSong Yang
Mar 3, 2018·Chemical Society Reviews·William M AumillerTrevor Douglas
May 2, 2018·Chemical Society Reviews·Yusuke AzumaDonald Hilvert
Feb 9, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Dan Stefan Petrescu, Amy Szuchmacher Blum
Feb 13, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Michael B DeciJuliane Nguyen
Oct 6, 2018·Biotechnology Journal·Sangwook ChuReza Ghodssi
Jun 21, 2017·Nature Chemical Biology·Christian B Rosen, Matthew B Francis
Oct 22, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Christina Wege, Claudia Koch
Nov 3, 2016·Nanotechnology·Serene S BayramAmy Szuchmacher Blum
Dec 27, 2017·Biotechnology Journal·Wenjing ZhangFeng Li
Sep 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·D VilonaM Bonchio
Sep 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu ZhangFei Wang
Jan 29, 2020·Proceedings of the National Academy of Sciences of the United States of America·Mark D LangowskiSheetij Dutta
Jun 10, 2020·Chemical Communications : Chem Comm·Xianxian MaoXiaojian Wang
Mar 1, 2016·Chemical Science·Valeria CastellettoMaxim G Ryadnov
Jun 1, 2010·Current Protocols in Chemical Biology·Leah S Witus, Matthew Francis
Nov 17, 2020·Frontiers in Chemistry·Wenbo ZhangChenxuan Wang
May 4, 2021·Journal of the American Chemical Society·Alexandra V RamseyMatthew B Francis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.