Nanoscale Structural Characterization of Individual Viral Particles Using Atomic Force Microscopy Infrared Spectroscopy (AFM-IR) and Tip-Enhanced Raman Spectroscopy (TERS)

Analytical Chemistry
Tianyi DouDmitry Kurouski

Abstract

Viruses are infections species that infect a large spectrum of living systems. Although displaying a wide variety of shapes and sizes, they are all composed of nucleic acid encapsulated into a protein capsid. After virions enter the host cell, they replicate to produce multiple copies of themselves. They then lyse the host, releasing virions to infect new cells. The high proliferation rate of viruses is the underlying cause of their fast transmission among living species. Although many viruses are harmless, some of them are responsible for severe diseases such as AIDS, viral hepatitis, and flu. Traditionally, electron microscopy is used to identify and characterize viruses. This approach is time- and labor-consuming, which is problematic upon pandemic proliferation of previously unknown viruses, such as H1N1 and COVID-19. Herein, we demonstrate a novel diagnosis approach for label-free identification and structural characterization of individual viruses that is based on a combination of nanoscale Raman and infrared spectroscopy. Using atomic force microscopy-infrared (AFM-IR) spectroscopy, we were able to probe structural organization of the virions of Herpes Simplex Type 1 viruses and bacteriophage MS2. We also showed that tip...Continue Reading

References

Dec 5, 1993·Journal of Molecular Biology·R GolmohammadiL Liljas
Sep 8, 2005·Journal of Clinical Microbiology·Jens DreierKnut Kleesiek
Nov 28, 2008·Journal of Virology·Zongli LiB V Venkataram Prasad
Nov 19, 2010·Journal of Virology·David SafronetzHeinz Feldmann
Jan 25, 2012·Journal of Biophotonics·Tanja Deckert-GaudigVolker Deckert
Jul 21, 2012·Journal of the American Chemical Society·Dmitry KurouskiIgor K Lednev
Feb 4, 2014·Journal of the American Chemical Society·Dmitry KurouskiLaurence A Nafie
Feb 20, 2014·Journal of the American Chemical Society·Jordan M KlingspornRichard P Van Duyne
May 24, 2015·Annual Review of Analytical Chemistry·Andrea Centrone
Dec 14, 2016·Chemical Reviews·Alexandre Dazzi, Craig B Prater
May 2, 2017·Chemical Reviews·Prabhat Verma
Jun 9, 2017·The Plant Pathology Journal·Jin-Sung Hong, Ho-Jong Ju
Jun 24, 2017·Chemical Society Reviews·Tanja Deckert-GaudigVolker Deckert
Oct 6, 2017·ACS Nano·Nicholas TallaridaVartkess Ara Apkarian
Oct 14, 2017·Analytical Chemistry·Lifu Xiao, Zachary D Schultz
Jan 9, 2018·Nanoscale·Alexander G MilekhinDietrich R T Zahn
Apr 7, 2018·Science·Shuai YuanXiangxi Wang
May 12, 2018·Angewandte Chemie·Ewelina LipiecRenato Zenobi
Feb 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Oliver W BayfieldAlfred A Antson
Jul 13, 2019·Chembiochem : a European Journal of Chemical Biology·Stanislav Rizevsky, Dmitry Kurouski
Jul 18, 2019·Nature Communications·Ran MengJunjie Zhang
Oct 16, 2019·ACS Applied Materials & Interfaces·Rui Wang, Dmitry Kurouski
Feb 23, 2020·Science·Daniel WrappJason S McLellan
May 20, 2020·Chemical Society Reviews·Dmitry KurouskiAndrea Centrone

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Citations

Apr 13, 2021·Frontiers in Bioengineering and Biotechnology·Maria Arista-RomeroLorenzo Albertazzi
May 21, 2021·Analytical Chemistry·Kaifeng ZhangTakehiro Tachizaki
Jul 3, 2021·Marine Drugs·Weimin Zuo, Hang Fai Kwok

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