Nanoscaled and microscaled parallel topography promotes tenogenic differentiation of ASC and neotendon formation in vitro

International Journal of Nanomedicine
Kaili ZhouWei Liu

Abstract

Topography at different scales plays an important role in directing mesenchymal stem cell differentiation including adipose-derived stem cells (ASCs) and the differential effect remains to be investigated. This study aimed to investigate the similarity and difference between micro- and nanoscaled aligned topography for inducing tenogenic differentiation of human ASCs (hASCs). Parallel microgrooved PDMS membrane and a parallel aligned electrospun nanofibers of gelatin/poly-ε-caprolactone mixture were employed as the models for the study. Aligned topographies of both microscales and nanoscales could induce an elongated cell shape with parallel alignment, as supported by quantitative cell morphology analysis (cell area, cell body aspect, and cell body major axis angle). qPCR analysis also demonstrated that the aligned topography at both scales could induce the gene expressions of various tenogenic markers at the 7th day of in vitro culture including tenomodulin, collagen I and collagen VI, decorin, tenascin-C and biglycan, but with upregulated expression of scleraxis and tenascin-C only in microscaled topography. Additionally, tenogenic differentiation at the 3rd day was confirmed only at microscale. Furthermore, microscaled topog...Continue Reading

Citations

Nov 6, 2018·Cellular and Molecular Life Sciences : CMLS·Jiayun HuangWeiliang Shen
Oct 17, 2018·International Journal of Molecular Sciences·Cristina SobacchiCiro Menale
Oct 14, 2020·Tissue Engineering. Part a·Aysegul Dede ErenJasper Foolen
Apr 28, 2021·ACS Biomaterials Science & Engineering·David H Ramos-RodriguezIlida Ortega Asencio
Nov 24, 2020·Nanomedicine : Nanotechnology, Biology, and Medicine·Aditi JainKaushik Chatterjee
Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Deepthi SankarJayakumar Rangasamy
Oct 24, 2021·International Journal of Biological Macromolecules·Burcu Sarıkaya, Menemşe Gümüşderelioğlu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

Image
Pro Plus
Mx3000PTM Real Time PCR System
SPSS
ImageJ

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.