Nanoscopic cell-wall architecture of an immunogenic ligand in Candida albicans during antifungal drug treatment

Molecular Biology of the Cell
Jia LinAaron K Neumann

Abstract

The cell wall of Candida albicans is composed largely of polysaccharides. Here we focus on β-glucan, an immunogenic cell-wall polysaccharide whose surface exposure is often restricted, or "masked," from immune recognition by Dectin-1 on dendritic cells (DCs) and other innate immune cells. Previous research suggested that the physical presentation geometry of β-glucan might determine whether it can be recognized by Dectin-1. We used direct stochastic optical reconstruction microscopy to explore the fine structure of β-glucan exposed on C. albicans cell walls before and after treatment with the antimycotic drug caspofungin, which alters glucan exposure. Most surface-accessible glucan on C. albicans yeast and hyphae is limited to isolated Dectin-1-binding sites. Caspofungin-induced unmasking caused approximately fourfold to sevenfold increase in total glucan exposure, accompanied by increased phagocytosis efficiency of DCs for unmasked yeasts. Nanoscopic imaging of caspofungin-unmasked C. albicans cell walls revealed that the increase in glucan exposure is due to increased density of glucan exposures and increased multiglucan exposure sizes. These findings reveal that glucan exhibits significant nanostructure, which is a previousl...Continue Reading

References

Sep 23, 1997·Cell·H J LoG R Fink
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Mar 17, 1999·FEMS Microbiology Letters·J M DaviesC A Gilligan
Apr 11, 2001·Emerging Infectious Diseases·R P Wenzel, M B Edmond
Aug 7, 2002·The Journal of Experimental Medicine·Gordon D BrownSiamon Gordon
Apr 30, 2003·The Journal of Experimental Medicine·Gordon D BrownSiamon Gordon
Feb 25, 2005·The EMBO Journal·Benjamin N GantnerDavid M Underhill
Sep 16, 2005·Drugs·Paul L McCormack, Caroline M Perry
May 3, 2006·PLoS Pathogens·Robert T Wheeler, Gerald R Fink
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Chad A RappleyeWilliam E Goldman
May 3, 2007·Protein Science : a Publication of the Protein Society·James BrownE Yvonne Jones
Jun 15, 2007·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Deverick J AndersonDaniel J Sexton
Jun 25, 2008·Infection and Immunity·Sarah E W GrubbMartin H Thornhill
Nov 7, 2009·FEMS Yeast Research·José Ruiz-Herrera, Lucila Ortiz-Castellanos
Apr 7, 2010·Nature Methods·Carlas S SmithKeith A Lidke
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·David AlsteensYves F Dufrêne
Nov 10, 2010·Antimicrobial Agents and Chemotherapy·Fernando C BizerraArnaldo L Colombo
Mar 17, 2011·PloS One·Melissa C GarciaPeter N Lipke
May 11, 2011·International Journal of Medical Microbiology : IJMM·Joachim F Ernst, Jesus Pla
May 12, 2011·Biomedical Optics Express·Fang HuangKeith A Lidke
Jul 2, 2011·Nature Protocols·Sebastian van de LindeMarkus Sauer
Jan 19, 2012·Infection and Immunity·Shih-Chin ChengMihai G Netea
Dec 12, 2012·Microbes and Infection·Kelly M ShepardsonRobert A Cramer
Dec 25, 2012·Nanoscale·Sofiane El-Kirat-ChatelYves F Dufrêne
Jan 26, 2013·Nature Protocols·Prabuddha SenguptaJennifer Lippincott-Schwartz
Apr 30, 2013·Nature Methods·Robert P J NieuwenhuizenBernd Rieger
Aug 9, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Steven de Jesus CarrionEric Pearlman
Feb 11, 2015·Journal of Biophotonics·Juliette GriffiéDylan M Owen
Oct 6, 2015·Nature Methods·Patrick Rubin-DelanchyDylan M Owen

❮ Previous
Next ❯

Citations

Sep 1, 2017·Molecular Biology of the Cell·Samantha L SchwartzDiane S Lidke
Jun 3, 2020·The Journal of Clinical Investigation·Anna VyborovaJürgen Kuball
Jul 26, 2018·Science Signaling·Arianne M BrandsmaJeanette H W Leusen
Jun 13, 2020·Frontiers in Cellular and Infection Microbiology·Louise A Walker, Carol A Munro
Feb 18, 2018·Trends in Microbiology·Alex HopkeRobert T Wheeler

❮ Previous
Next ❯

Methods Mentioned

BETA
optical microscopy
confocal microscopy
fluorescence imaging

Software Mentioned

SET
DBSCAN
dSTORM
MATLAB

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

The Journal of Infectious Diseases
Joseph B LillegardThomas R Kozel
Trends in Immunology
Sigrid E M HeinsbroekSiamon Gordon
Proceedings of the Society for Experimental Biology and Medicine
R A VOGEL
© 2022 Meta ULC. All rights reserved