Nanoscopic X-ray tomography for correlative microscopy of a small meiofaunal sea-cucumber.

Scientific Reports
Simone FerstlFranz Pfeiffer

Abstract

In the field of correlative microscopy, light and electron microscopy form a powerful combination for morphological analyses in zoology. Due to sample thickness limitations, these imaging techniques often require sectioning to investigate small animals and thereby suffer from various artefacts. A recently introduced nanoscopic X-ray computed tomography (NanoCT) setup has been used to image several biological objects, none that were, however, embedded into resin, which is prerequisite for a multitude of correlative applications. In this study, we assess the value of this NanoCT for correlative microscopy. For this purpose, we imaged a resin-embedded, meiofaunal sea cucumber with an approximate length of 1 mm, where microCT would yield only little information about the internal anatomy. The resulting NanoCT data exhibits isotropic 3D resolution, offers deeper insights into the 3D microstructure, and thereby allows for a complete morphological characterization. For comparative purposes, the specimen was sectioned subsequently to evaluate the NanoCT data versus serial sectioning light microscopy (ss-LM). To correct for mechanical instabilities and drift artefacts, we applied an alternative alignment procedure for CT reconstruction....Continue Reading

References

Nov 24, 2007·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·M BechF Pfeiffer
Jun 26, 2008·Histochemistry and Cell Biology·Ben N G Giepmans
Feb 25, 2009·Medical Physics·V PatelS Rudin
Apr 28, 2009·Journal of Synchrotron Radiation·P KraftB Schmitt
Jul 26, 2011·Current Opinion in Structural Biology·Jeffrey CaplanKirk J Czymmek
Dec 14, 2011·Journal of Structural Biology·Dilworth Y ParkinsonMark A Le Gros
Nov 25, 2014·Biophysical Journal·Elizabeth A SmithCarolyn A Larabell
Mar 7, 2017·Molecular Phylogenetics and Evolution·Allison K MillerGreg W Rouse
Nov 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Mark MüllerFranz Pfeiffer
Dec 15, 2017·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Timur E GureyevHarry M Quiney
Feb 23, 2018·Journal of Microscopy·Henry JahnJörg U Hammel
May 22, 2019·Zoological Letters·Vladimir GrossFranz Pfeiffer

❮ Previous
Next ❯

Methods Mentioned

BETA
correlative
imaging techniques
electron microscopy
super-resolution
scanning
transmission electron microscopy
X-ray
correlative microscopy
light microscopy

Software Mentioned

Register Images
NanoCT
Photoshop
Amira
AlignSlices

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.