Nanosecond electric pulse-induced calcium entry into chromaffin cells

Bioelectrochemistry
P Thomas VernierGale L Craviso

Abstract

Electrically excitable bovine adrenal chromaffin cells were exposed to nanosecond duration electric pulses at field intensities ranging from 2 MV/m to 8 MV/m and intracellular calcium levels ([Ca(2+)](i)) monitored in real time by fluorescence imaging of cells loaded with Calcium Green. A single 4 ns, 8 MV/m pulse produced a rapid, short-lived increase in [Ca(2+)](i), with the magnitude of the calcium response depending on the intensity of the electric field. Multiple pulses failed to produce a greater calcium response than a single pulse, and a short refractory period was required between pulses before another maximal increase in [Ca(2+)](i) could be triggered. The pulse-induced rise in [Ca(2+)](i) was not affected by depleting intracellular calcium stores with caffeine or thapsigargin but was completely prevented by the presence of EGTA, Co(2+), or the L-type calcium channel blocker nitrendipine in the extracellular medium. Thus, a single nanosecond pulse is sufficient to elicit a rise in [Ca(2+)](i) that involves entry of calcium via L-type calcium channels.

References

Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Apr 1, 1983·Journal of Neuroscience Methods·J C WaymireJ W Haycock
Sep 6, 2001·Bioelectromagnetics·K H SchoenbachE S Buescher
Oct 3, 2003·Biochemical and Biophysical Research Communications·P Thomas VernierMartin A Gundersen
Dec 4, 2003·Bioelectrochemistry·Pamela S HairE Stephen Buescher
Mar 18, 2004·The Journal of Biological Chemistry·Jody A WhiteStephen J Beebe
Sep 24, 2004·Physiological Measurement·Stephen J BeebeKarl H Schoenbach
Jan 26, 2006·IEEE Transactions on Nanobioscience·Yinghua SunMartin A Gundersen
Mar 21, 2006·Biochemical and Biophysical Research Communications·Richard NuccitelliKarl H Schoenbach
May 6, 2006·Journal of Biomedical Optics·Yinghua SunLaura Marcu
Oct 4, 2006·Physiological Reviews·Antonio G GarcíaJavier García-Sancho
Oct 21, 2006·BMC Cell Biology·P Thomas VernierMartin A Gundersen
Apr 10, 2007·International Journal of Cancer. Journal International Du Cancer·Edward B GaronH Phillip Koeffler

❮ Previous
Next ❯

Citations

Jul 8, 2010·The Journal of Membrane Biology·Stefania RomeoLuigi Zeni
Apr 3, 2010·Medical & Biological Engineering & Computing·Tina Batista NapotnikDamijan Miklavcic
Jun 23, 2011·PloS One·Charlotte S Daniels, Boris Rubinsky
Oct 22, 2014·Apoptosis : an International Journal on Programmed Cell Death·Larry E EstlackBennett L Ibey
Dec 27, 2011·Bioelectrochemistry·Gale L CravisoP Thomas Vernier
Jul 1, 2014·Biochimica Et Biophysica Acta·Olga N PakhomovaAndrei G Pakhomov
Apr 19, 2013·The Journal of Membrane Biology·Agnese DenziMicaela Liberti
May 2, 2009·International Journal of Cancer. Journal International Du Cancer·Richard NuccitelliKarl H Schoenbach
Aug 4, 2011·Bioelectromagnetics·Marie Breton, Lluis M Mir
Jun 10, 2014·Clinical and Experimental Pharmacology & Physiology·Nai-Jung ChiangLi-Tzong Chen
Feb 1, 2011·IEEE Transactions on Bio-medical Engineering·Delia Arnaud-CormosP Thomas Vernier
Jun 14, 2012·IEEE Transactions on Bio-medical Engineering·C MerlaM Liberti
Feb 17, 2009·Biophysical Journal·Sufen WangMiguel Valderrábano
Dec 12, 2012·Biochimica Et Biophysica Acta·Iurii SemenovAndrei G Pakhomov
Mar 19, 2013·Biochimica Et Biophysica Acta·Stefania RomeoP Thomas Vernier
May 27, 2015·Bioelectrochemistry·Iurii SemenovAndrei G Pakhomov
Sep 6, 2014·Bioelectrochemistry·Louise Chopinet, Marie-Pierre Rols
Sep 10, 2014·Bioelectrochemistry·Gale L CravisoP Thomas Vernier
Dec 18, 2016·Cellular and Molecular Life Sciences : CMLS·Elena C GianulisAndrei G Pakhomov
Feb 13, 2016·PloS One·Michael K StehlingBoris Rubinsky
Jan 1, 2010·Cancers·Stephen J BeebeRichard Heller
Jun 6, 2018·IEEE Transactions on Nanobioscience·Patrizia LambertiMichele Compitiello
May 24, 2018·BioMed Research International·Tarique R BagalkotGale L Craviso
May 15, 2020·International Journal of Molecular Sciences·Wenfei BoAndrei G Pakhomov
Jul 28, 2020·Bioelectrochemistry·Andrei G Pakhomov, Olga N Pakhomova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical and Biophysical Research Communications
Andrei G PakhomovKarl H Schoenbach
Biochemical and Biophysical Research Communications
P Thomas VernierMartin A Gundersen
Bioelectromagnetics
Karl H SchoenbachE Stephen Buescher
© 2022 Meta ULC. All rights reserved