Nanosecond laser ablation enhances cellular infiltration in a hybrid tissue scaffold

Materials Science & Engineering. C, Materials for Biological Applications
S JahnaviR S Verma

Abstract

Hybrid tissue engineered (HTE) scaffolds constituting polymeric nanofibers and biological tissues have attractive bio-mechanical properties. However, they suffer from small pore size due to dense overlapping nanofibers resulting in poor cellular infiltration. In this study, using nanosecond (ns) laser, we fabricated micro-scale features on Polycaprolactone (PCL)-Chitosan (CH) nanofiber layered bovine pericardium based Bio-Hybrid scaffold to achieve enhanced cellular adhesion and infiltration. The laser energy parameters such as fluence of 25J/cm(2), 0.1mm instep and 15 mark time were optimized to get structured microchannels on the Bio-Hybrid scaffolds. Laser irradiation time of 40μs along with these parameters resulted in microchannel width of ~50μm and spacing of ~35μm between adjacent lines. The biochemical, thermal, hydrophilic and uniaxial mechanical properties of the Bio-Hybrid scaffolds remained comparable after laser ablation reflecting extracellular matrix (ECM) stability. Human umbilical cord mesenchymal stem cells and mouse cardiac fibroblasts seeded on these laser-ablated Bio-Hybrid scaffolds exhibited biocompatibility and increased cellular adhesion in microchannels when compared to non-ablated Bio-Hybrid scaffolds...Continue Reading

Citations

Jul 3, 2021·Materials·Alan F Murray, Evangelos Delivopoulos
Nov 18, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Atsushi MaharaTetsuji Yamaoka

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