Nanosecond pulsed electric fields (nsPEFs) activate intrinsic caspase-dependent and caspase-independent cell death in Jurkat cells

Biochemical and Biophysical Research Communications
Wei RenStephen J Beebe

Abstract

NsPEF ablation induces apoptosis markers, but specific cell death pathways have not been fully defined. To identify nsPEF-activated cell death pathways, wildtype human Jurkat cells and clones with deficiencies in extrinsic and intrinsic apoptosis pathways were investigated. NsPEFs activated caspase isozymes and induced identical electric field-dependent cell death in clones deficient in FADD or caspase-8, indicating that extrinsic apoptosis pathways were not activated. This was confirmed when cytochrome c release was shown to be unaffected by the pan caspase inhibitor, z-VAD-fmk. NsPEF-treated APAF-1-silenced cells did not exhibit caspase-3/7 and -9 activities and corresponding electric field-dependent cell death in this clone was attenuated compared to its vector control at low, but not at high electric fields. These data demonstrate that nsPEFs induce intrinsic apoptosis activate by cytochrome c release from mitochondria through an APAF-1- and caspase-dependent pathway as well as through caspase-independent mechanisms that remain to be defined. Furthermore, the results establish that nsPEFs can overcome natural and oncogenic mechanisms that promote cell survival through inhibition of apoptosis and other cell death mechanisms.

References

Jul 31, 1999·The Journal of Biological Chemistry·C ScaffidiM E Peter
Sep 6, 2001·Bioelectromagnetics·K H SchoenbachE S Buescher
Sep 5, 2002·European Journal of Immunology·Cédric VonarbourgFrédéric Rieux-Laucat
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephen J BeebeKarl H Schoenbach
Oct 3, 2003·Biochemical and Biophysical Research Communications·P Thomas VernierMartin A Gundersen
Oct 9, 2003·The Journal of Biological Chemistry·Francis Ka-Ming ChanMichael J Lenardo
Jul 15, 2005·Nature Medicine·Guido Kroemer, Seamus J Martin
Feb 14, 2006·Biochemical and Biophysical Research Communications·Thiruvallur R GowrishankarJames C Weaver
Mar 21, 2006·Biochemical and Biophysical Research Communications·Richard NuccitelliKarl H Schoenbach
Apr 10, 2007·International Journal of Cancer. Journal International Du Cancer·Edward B GaronH Phillip Koeffler
May 24, 2007·Apoptosis : an International Journal on Programmed Cell Death·Emily H HallStephen J Beebe
Oct 18, 2008·The Journal of Biological Chemistry·Mary E ShawgoJohn D Robertson
May 2, 2009·International Journal of Cancer. Journal International Du Cancer·Richard NuccitelliKarl H Schoenbach
Sep 18, 2009·The Journal of Biological Chemistry·Mary E ShawgoJohn D Robertson
Apr 8, 2010·Pigment Cell & Melanoma Research·Xinhua ChenStephen J Beebe
Oct 28, 2010·The Journal of Biological Chemistry·Shary N SheltonJohn D Robertson
Jan 8, 2011·Apoptosis : an International Journal on Programmed Cell Death·Wei Ren, Stephen J Beebe
Jan 21, 2011·Cancer Metastasis Reviews·Oliver KeppGuido Kroemer

❮ Previous
Next ❯

Citations

Aug 1, 2013·Biochemical and Biophysical Research Communications·Keiko Morotomi-YanoKen-ichi Yano
Jul 31, 2013·PloS One·Olga N PakhomovaAndrei G Pakhomov
Sep 17, 2013·International Journal of Nanomedicine·Stephen J Beebe
Oct 22, 2014·Apoptosis : an International Journal on Programmed Cell Death·Larry E EstlackBennett L Ibey
Nov 8, 2014·Cancer Cell International·Shan WuStephen J Beebe
Jul 1, 2014·Biochimica Et Biophysica Acta·Olga N PakhomovaAndrei G Pakhomov
Aug 2, 2014·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Ru ChenStephen J Beebe
May 18, 2013·Biochemical and Biophysical Research Communications·Richard NuccitelliPamela Nuccitelli
Sep 6, 2014·Bioelectrochemistry·Louise Chopinet, Marie-Pierre Rols
Nov 12, 2016·Scientific Reports·Claudia MuratoriOlga N Pakhomova
Dec 18, 2016·Cellular and Molecular Life Sciences : CMLS·Elena C GianulisAndrei G Pakhomov
Sep 26, 2017·International Journal of Cancer. Journal International Du Cancer·Siqi GuoStephen J Beebe
Feb 21, 2019·Annual Review of Biophysics·Tadej KotnikDamijan Miklavčič
Feb 7, 2020·Annals of Biomedical Engineering·Borja MercadalAntoni Ivorra
Mar 1, 2017·The Journal of Membrane Biology·Claudia MuratoriOlga N Pakhomova
Jul 7, 2017·FEBS Open Bio·Keiko Morotomi-Yano, Ken-Ichi Yano
Jan 25, 2019·Scientific Reports·Alessandra RossiClaudia Muratori
Jun 4, 2014·Archives of Biochemistry and Biophysics·Keiko Morotomi-YanoKen-ichi Yano
Jun 20, 2021·Bioelectrochemistry·Tina Batista NapotnikDamijan Miklavčič

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Apoptosis : an International Journal on Programmed Cell Death
Wei Ren, Stephen J Beebe
Hepatobiliary & Pancreatic Diseases International : HBPD INT
Xinhua ChenShu-Sen Zheng
Biochemical and Biophysical Research Communications
Andrei G PakhomovKarl H Schoenbach
© 2021 Meta ULC. All rights reserved